中国动物检疫2026，Vol.43Issue(8)：30-40,11.DOI:10.3969/j.issn.1005-944X.2026.08.005
山东省济宁市部分鹅场舍内空气细菌检测及耐药基因分析
Detection of Airborne Bacteria and Analysis of Antimicrobial Resistance Genes in Goose Farm Barns in Jining,Shandong
倪伟 1赵翠 2伊惠 2李金晔 3闫超 2樊丽 2李宁 3李玉强 2于鲁娜3
作者信息
- 1. 新泰市畜牧兽医事业发展服务中心,山东新泰 271200
- 2. 泰安市畜牧兽医事业发展服务中心,山东 泰安 271000
- 3. 山东农业大学动物医学院,山东 泰安 271000
- 折叠
摘要
Abstract
Microbial aerosols originating from livestock and poultry farms are major reservoirs for pathogenic microorganisms and antimicrobial resistance genes(ARGs),posing a serious threat to the health of both animals and farm workers.However,limited information has been reported on the composition of aerosol microbial communities within goose barns and their associated ARGs.In this study,airborne bacterial community composition and associated ARG profiles were analyzed using metagenomic sequencing and PCR,targeting two gosling farms(geese aged 10 days)and two growing goose farms(geese aged 50 to 55 days)in Jining,Shandong Province.The results showed that at the phylum level,the airborne bacterial communities were dominated by Proteobacteria and Firmicutes;at the genus level,by Psychrobacter,Corynebacterium,Aerococcus and Staphylococcus;and at the species level,multiple zoonotic opportunistic pathogens were detected,including Aerococcus urinaeequi,Mammaliicoccus sciuri,and Streptococcus alactolyticus.Metagenomic analysis identified a total of 1 424 ARG subtypes,predominantly conferring resistance to fluoroquinolones,tetracyclines,and macrolides.A total of 36 ARG subtypes were tested as positive by a targeted PCR assay on culturable airborne bacteria,covering nine major classes of antibiotics(e.g.,β-lactams and quinolones),and ARG profiles differed among the goose barns.The composition of airborne microbial communities and the characteristics of ARG contamination in goose barns were preliminarily revealed,providing baseline data for evaluating biosafety risks in waterfowl production environments and formulating scientific prevention and control strategies.关键词
鹅舍/微生物气溶胶/抗生素耐药基因/宏基因组学Key words
goose barn/microbial aerosol/antimicrobial resistance gene/metagenomics分类
农业科技引用本文复制引用
倪伟,赵翠,伊惠,李金晔,闫超,樊丽,李宁,李玉强,于鲁娜..山东省济宁市部分鹅场舍内空气细菌检测及耐药基因分析[J].中国动物检疫,2026,43(8):30-40,11.
