中国美容医学2026，Vol.35Issue(9)：35-40,6.
脱细胞真皮基质联合富血小板血浆修复兔全层皮肤缺损的疗效及机制研究
Therapeutic Efficacy and Mechanism of Acellular Dermal Matrix Combined with Platelet-rich Plasma in Repairing Full-thickness Skin Defects in Rabbits
摘要
Abstract
Objective To investigate the therapeutic efficacy and underlying mechanism of acellular dermal matrix(ADM)combined with platelet-rich plasma(PRP)in the repair of full-thickness skin defects in rabbits.Methods Twelve healthy New Zealand white rabbits were used to establish 72 full-thickness skin defect models on the dorsal region and were randomly divided into three groups:ADM+PRP group,ADM group,and blank control group.Macroscopic observation,hematoxylin-eosin(HE)staining,picrosirius red staining,and immunohistochemical detection of TGF-β1 and VEGF expression were performed immediately postoperatively and at 1,2,3,and 4 weeks.Images were collected and statistically analyzed.Results The ADM+PRP group showed significantly better wound healing than the ADM and control groups.On day 7,obvious granulation tissue formation and wound contraction were observed;by day 14,most wounds had closed;and by day 28,wounds were nearly completely healed,with good hair growth and minimal scarring.The ADM group exhibited delayed healing,with necrotic tissue remaining on day 14 and incomplete healing by day 28.The control group showed slow healing throughout,with residual wounds still present at day 28.Wound healing rates were significantly higher in the ADM+PRP group at all time points(P＜0.05).At week 2,granulation tissue in the ADM+PRP group exhibited a characteristic"crater-like"morphology,indicating active repair.By week 4,collagen deposition and tissue remodeling were more pronounced in this group.Picrosirius red staining and immunohistochemistry showed that at weeks 2 and 4,the ADM+PRP group had significantly higher levels of type Ⅰ and Ⅲ collagen,a higher proportion of type Ⅰ collagen,and increased expression of VEGF and TGF-β1 compared to the other groups(P＜0.05).Conclusion ADM combined with PRP significantly promotes the healing of full-thickness skin defects by enhancing granulation tissue formation,collagen deposition,and the expression of angiogenic factors VEGF and TGF-β1,leading to improved skin repair quality.This approach is superior to ADM alone and holds promising potential for clinical application.关键词
脱细胞真皮基质/富血小板血浆/皮肤缺损/创面修复/转化生长因子β1(TGF-β1)/血管内皮生长因子(VEGF)Key words
acellular dermal matrix/platelet-rich plasma/skin defect/wound healing/transforming growth factor-β1(TGF-β1)/vascular endothelial growth factor(VEGF)分类
医药卫生引用本文复制引用
钱莉丽,曹咬脐,刘泾科,王悦奇,熊亮..脱细胞真皮基质联合富血小板血浆修复兔全层皮肤缺损的疗效及机制研究[J].中国美容医学,2026,35(9):35-40,6.基金项目
苏州市吴江区科教兴卫项目(编号:WWK202216) （编号:WWK202216）
