中国农业科学2026，Vol.59Issue(18)：4021-4032,12.DOI:10.3864/j.issn.0578-1752.2026.18.006
冷藏和杀胚处理对米蛾卵扩繁松毛虫赤眼蜂效率的影响
Effects of Refrigeration and Embryo-Killing Treatment of Corcyra cephalonica Eggs on Propagation Efficiency of Egg Parasitoid Trichogramma dendrolimi
摘要
Abstract
[Background]Efficient artificial propagation of egg parasitoids requires high quality alternative host eggs,so the fresh and nutritional states of host eggs are key factors to the propagation efficiency of egg parasitoids.[Objective]The Trichogramma dendrolimi,an egg parasitoid widely used in agricultural production,and its dominant alternative host Corcyra cephalonica eggs were used as test materials to explore the effects of refrigeration and embryo-killing on the quality of host eggs,so as to provide data support for scientific preservation of host eggs,optimization of artificial propagation technology of egg parasitoids,improvement of propagation efficiency and reduction of control costs.[Method]Four treatments of 1-day-old C.cephalonica eggs were performed:4℃ refrigeration for 3 d followed by UV irradiation for embryo-killing(3 d+K),UV irradiation followed by 4℃ storage for 3 d(K+3 d),4℃ storage for 7 d followed by UV irradiation(7 d+K),and UV irradiation followed by 4℃ storage for 7 d(K+7 d),with eggs only treated with UV irradiation for embryo-killing(K)as control.The parasitism rate of T.dendrolimi and the eclosion rate,female ratio,and developmental duration of the offspring parasitoids in eggs resulted from different treatments were compared.The contents of triglyceride,total protein,and trehalose in C.cephalonica eggs from each treatment were quantified,as well as the Wolbachia levels in both C.cephalonica eggs and T.dendrolimi emerged from the eggs,and their correlations with the biological indices of T.dendrolimi were analyzed.[Result]Compared with the control(K),the 7 d+K treatment significantly increased about 20％ of the parasitism rate,and significantly increased about 72％ of the offspring female amount of T.dendrolimi,without significant impact on eclosion rate and developmental duration of the offspring.The K+7 d treatment also could significantly increase 19％ parasitism rate of the T.dendrolimi,but decreased the eclosion rate of the offspring wasps.Nutrient analysis showed that 4℃ storage(either before or after embryo-killing)significantly increased the triglyceride content in C.cephalonica eggs.Refrigeration treatment for 3 d before UV irradiation(3 d+K)significantly increased the protein content.None of the treatments significantly affected the trehalose content in host eggs.Correlation analysis revealed that triglyceride content in the eggs was significantly positively correlated with the parasitism rate of T.dendrolimi,the female proportion of its offspring and the Wolbachia level in the offspring wasps.[Conclusion]Pretreatment of 1-day-old C.cephalonica eggs with 7 d of refrigeration at 4℃ prior to embryo-killing under UV can effectively extend the duration of their freshness.This pretreatment may promote the parasitism efficiency and offspring female ratio of T.dendrolimi by increasing nutrient content,such as triglyceride,in host eggs.This study provides an optimized preservation and processing protocol for efficient and low-cost artificial mass rearing of T.dendrolimi using C.cephalonica eggs as an alternative host.关键词
松毛虫赤眼蜂/米蛾卵/冷藏/甘油三酯/人工扩繁Key words
Trichogramma dendrolimi/Corcyra cephalonica egg/refrigeration/triglyceride/artificial mass rearing引用本文复制引用
孙佳宁,刘晨曦,刘小侠,李贞..冷藏和杀胚处理对米蛾卵扩繁松毛虫赤眼蜂效率的影响[J].中国农业科学,2026,59(18):4021-4032,12.基金项目
国家重点研发计划(2023YFE0123000)、国家现代农业产业技术体系建设专项资金(CARS-28) （2023YFE0123000）
