中国农业科学2026，Vol.59Issue(18)：4048-4055,8.DOI:10.3864/j.issn.0578-1752.2026.18.008
斜纹夜蛾对乙基多杀菌素的抗性监测及抗性遗传方式
Resistance Monitoring and Inheritance of Resistance to Spinetoram in Spodoptera litura
摘要
Abstract
[Objective]This study aims to clarify the current resistance status and underlying mechanisms of field populations of Spodoptera litura to spinetoram,and to provide a scientific basis for field resistance monitoring and the development of integrated resistance management(IRM)strategies.[Method]From 2023 to 2024,23 field populations of S.litura were collected across China.The resistance levels to spinetoram were determined using the leaf-dipping method.The highly resistant Nanning(NN)population and the susceptible strain(SS)were subjected to reciprocal crosses and mass crosses,and the F1 progeny larvae were bioassayed to analyze the resistance inheritance pattern.The synergistic effects of piperonyl butoxide(PBO),diethyl maleate(DEM),and triphenyl phosphate(TPP)were evaluated,and the activities of carboxylesterase(CarE),glutathione S-transferase(GST),and cytochrome P450 monooxygenase(P450)were measured in both the NN and SS strains.[Result]Field resistance monitoring revealed significant variation in spinetoram resistance among different geographic populations of S.litura.High-level resistance(43.3-to 107.8-fold)was detected in populations from Nanning(Guangxi)and Fuzhou(Fujian)in 2023,and from Sanya(Hainan),Kunming(Yunnan),Weifang(Shandong),and Xuchang(Henan)in 2024.Notably,the resistance ratios for the Nanning(2023)and Sanya(2024)populations reached 106.8-and 107.8-fold,respectively.Moderate resistance levels were observed in the 2023 Ningbo(Zhejiang)and Jingzhou(Hubei)populations,as well as the 2024 Fuzhou(Fujian),Jingzhou(Hubei),Guangzhou(Guangdong),Changsha(Hunan),Nanjing(Jiangsu),Shanghai,and Guilin(Guangxi)populations,with resistance ratios ranging from 10.3-to 31.8-fold.Low resistance levels were found in the 2023 Hangzhou(Zhejiang)population and the 2024 Nanchang(Jiangxi),Hefei(Anhui),Beijing,and Hangzhou(Zhejiang)populations,with resistance ratios between 5.0-and 8.9-fold.Inheritance analysis showed that resistance to spinetoram in the NN population was controlled by an autosomal,incompletely recessive gene.The synergists DEM and TPP exhibited synergistic ratios of 1.8 and 1.5,respectively,suppressing resistance in the NN population.Moreover,the activities of GST and CarE in the NN population were 4.1-and 3.5-fold higher than those in the susceptible strain,respectively.[Conclusion]Resistance to spinetoram has developed in S.litura populations across multiple regions.Enhanced activities of GST and CarE play important roles in the resistance of S.litura to spinetoram.关键词
斜纹夜蛾/乙基多杀菌素/抗性监测/不完全隐性遗传/增效作用/抗性治理Key words
Spodoptera litura/spinetoram/resistance monitoring/incompletely recessive inheritance/synergism/resistance management引用本文复制引用
李皓,高新菊,卢鹏,詹千缘,王然,苏奇,渠成..斜纹夜蛾对乙基多杀菌素的抗性监测及抗性遗传方式[J].中国农业科学,2026,59(18):4048-4055,8.基金项目
国家特色蔬菜产业技术体系(CARS-24-C-03) （CARS-24-C-03）
