中国普通外科杂志2026，Vol.35Issue(7)：1364-1375,12.DOI:10.7659/j.issn.1005-6947.260234
NUDT1在肝细胞癌中的表达特征、预后价值及促癌作用机制研究
Expression characteristics,prognostic significance,and tumor-promoting mechanisms of NUDT1 in hepatocellular carcinoma
摘要
Abstract
Background and Aims:Hepatocellular carcinoma(HCC)is characterized by high malignancy,invasiveness,and recurrence rates.Identification of novel molecular biomarkers and therapeutic targets is essential for improving patient outcomes.Nudix hydrolase 1(NUDT1),a key regulator involved in oxidative stress response and DNA damage repair,has been reported to be dysregulated in various cancers;however,its role in HCC remains unclear.This study aimed to investigate the expression pattern,prognostic significance,and biological function of NUDT1 in HCC. Methods:RNA sequencing data from The Cancer Genome Atlas(TCGA),Gene Expression Omnibus(GEO),and clinical samples were analyzed using bioinformatics approaches to identify HCC-associated genes.The expression level,diagnostic performance,and prognostic value of NUDT1 were evaluated.Immunohistochemistry was performed on 60 paired HCC and adjacent normal tissues to validate NUDT1 expression and analyze its association with clinicopathological characteristics and survival outcomes.Stable NUDT1-knockdown HepG2 cells and NUDT1-overexpressing LM3 cells were established.Cell proliferation,colony formation,migration,invasion,and apoptosis were evaluated using the CCK-8 assay,colony formation assay,wound healing assay,Transwell assay,and flow cytometry.The involvement of the PI3K/Akt signaling pathway was further examined using pathway agonists and inhibitors. Results:NUDT1 was significantly upregulated in HCC tissues and was associated with poor overall survival,disease-specific survival,and progression-free interval.Immunohistochemical analysis confirmed increased NUDT1 protein expression in HCC tissues,which was correlated with TNM stage,tumor size,and vascular invasion.Functional assays demonstrated that NUDT1 knockdown inhibited HCC cell proliferation,migration,and invasion while promoting apoptosis,whereas NUDT1 overexpression exerted opposite effects.Mechanistically,activation of the PI3K/Akt pathway partially reversed the inhibitory effects induced by NUDT1 knockdown,while PI3K/Akt inhibition attenuated the tumor-promoting effects of NUDT1 overexpression. Conclusion:NUDT1 is highly expressed in HCC and associated with poor prognosis.NUDT1 promotes HCC progression through activation of the PI3K/Akt signaling pathway and may serve as a potential prognostic biomarker and therapeutic target.关键词
癌,肝细胞/Nudix水解酶/预后/细胞增殖/肿瘤浸润Key words
Carcinoma,Hepatocellular/Nudix Hydrolases/Prognosis/Cell Proliferation/Neoplasm Invasiveness分类
医药卫生引用本文复制引用
陈勇治,彭睿,周宁,刘韦男,王冠武,刘文,李嘉欣,黄怀银..NUDT1在肝细胞癌中的表达特征、预后价值及促癌作用机制研究[J].中国普通外科杂志,2026,35(7):1364-1375,12.基金项目
湖南省教育厅科学研究基金资助项目(25C0006,23B0055). （25C0006,23B0055）
