中国水稻科学2026，Vol.40Issue(5)：607-617,11.DOI:10.16819/j.1001-7216.2026.250909
水稻颖壳发育基因AGL1的图位克隆与功能分析
Map-based Cloning and Functional Characterization of AGL1,a Gene Regulating Lemma and Palea Development in Rice
摘要
Abstract
[Objective]Rice lemma and palea development directly affects grain shape,pollination efficiency,yield and quality.This study identified mutants related to rice glume development,mapped and cloned the causal genes,and elucidated the molecular mechanisms by which the target genes regulate glume development,providing theoretical references for rice molecular breeding.[Methods]The agl1 mutant was obtained by EMS mutagenesis of japonica cultivar SN9816.Paraffin sectioning and scanning electron microscopy were used to observe the morphology of glumes and floral organs;pollen viability was assessed by I₂-KI staining.Map-based cloning delimited AGL1,candidate genes were sequenced,and conservation and diversity of the target gene were analyzed.A Foss grain analyzer was used to measure brown-rice quality parameters.[Results]Compared with the wild-type SN9816,agl1 showed more strongly curved lemmas with unchanged longitudinal length,inwardly sunken paleae,and significantly narrowed lemma and palea;the lemma almost completely enclosed the palea.agl1 displayed abnormal lodicules,enlarged pistils and disordered stamens.Pollen activity and seed-setting rate were reduced.AGL1 was fine-mapped to a 68.7-kb interval between Indel4 and Indel5 on chromosome 2.Sequencing seven ORFs in this region revealed a single-nucleotide mutation in LOC_Os02g56610,which encodes a DUF640-domain protein.The DUF640 family in rice harbors a conserved ALOG domain and diverse regulatory elements,suggesting roles in both floral morphogenesis and grain quality.Quality analysis showed increased brown-rice protein and free fatty acid content but decreased amylose and total starch content,leading to poorer eating quality.[Conclusion]The AGL1 gene functions as a pivotal regulator of rice lemma and palea development and grain quality;its loss-of-function disrupts hull morphogenesis,compromises pollen viability,and likely perturbs the grain-filling process,resulting in the concomitant deterioration of both yield and grain quality.关键词
水稻畸形颖壳/籽粒形态/花粉活性/图位克隆/食味品质/DUF640家族Key words
aberrant rice lemma/grain shape/pollen viability/map-based cloning/eating quality/DUF640 family引用本文复制引用
冯莆淋,杨绮文,吴佳益,范鸣谦,程艳双,邱忠华,郭然,陈温福,王嘉宇..水稻颖壳发育基因AGL1的图位克隆与功能分析[J].中国水稻科学,2026,40(5):607-617,11.基金项目
生物育种国家科技重大专项(2023ZD04022) （2023ZD04022）
沈阳市自然科学基金(基础研究)资助项目(23-503-6-20). （基础研究）
