中国饲料Issue(16)：10-16,7.DOI:10.15906/j.cnki.cn11-2975/s.20261602
牦牛瘤胃厌氧真菌木聚糖酶基因工程菌的构建及其酶学特性研究
Construction of a genetically engineered strain for xylanase from yak rumen anaerobic fungus and characterization of its enzymatic properties
摘要
Abstract
To construct an engineered strain P.p(xyn-N.f)using the xylanase gene from the rumen anaerobic fungus Piromyces YakTz of Tianzhu yak.The β-1,4-endoxylanase gene was cloned from this fungus,codon-optimized,and used to generate a recombinant Pichia pastoris strain for inducible expression.Enzyme activity and enzymatic properties were determined by visible spectrophotometry.Results:(1)The xylanase produced by the genetically engineered strain P.p(xyn-N.f)exhibited an optimal temperature range of 40～60℃,an optimal pH value range of 5.0～9.0.(2)Mn2+,Fe2+,Hg2+,K+,Zn2+inhibited xylanase activity;Ba2+,Co2+had no significant effect;Cu2+,Mg2+enhanced its activity.(3)After 144 h of induction in aerobic shake flasks,xylanase activity reached 55.95 IU/mL.Upon scale-up cultivation in a 15 L fermenter,the activity increased to 500.70 IU/mL.Conclusion:This study successfully achieved high-level expression of the engineered strain P.p(xyn-N.f)under aerobic conditions.The enzyme produced shows high activity,favorable thermal stability,and broad pH adaptability,and holds promise for application in the feed industry.关键词
牦牛/瘤胃厌氧真菌/木聚糖酶/基因工程菌/酶学特性Key words
yak/rumen anaerobic fungus/xylanase/genetically engineered strain/enzymatic properties分类
农业科技引用本文复制引用
魏亚琴,赵疆,郑群,许哲,吴倩曼..牦牛瘤胃厌氧真菌木聚糖酶基因工程菌的构建及其酶学特性研究[J].中国饲料,2026,(16):10-16,7.基金项目
甘肃省科学院重点研发项目(2024ZDYF—02) （2024ZDYF—02）
甘肃省科学院青年基金项目(2022QN—02) （2022QN—02）
国家自然科学基金-地区基金项目(32660962) （32660962）
