中国饲料Issue(17)：15-22,8.DOI:10.15906/j.cnki.cn11-2975/s.2025040010-08
绵羊肌肉发育关键基因KLF5的CRISPR/Cas9编辑及对产肉性状的调控机制
CRISPR/Cas9 editing of KLF5,a key gene in sheep muscle development,and its regulatory mechanisms for meat production traits
摘要
Abstract
KLF5(Krüppel-like factor 5),as a member of the transcription factor family,plays an important role in cell proliferation,differentiation,and tissue development.This study targeted the editing of the sheep KLF5 gene using CRISPR/Cas9 technology to explore its regulatory mechanism on muscle cell proliferation,differentiation,and meat production traits.Experimental results indicated that KLF5 knockout significantly promote the proliferation and differentiation capacity of sheep muscle cells and upregulate the expression of muscle development-related genes(such as MYOD and MYOG).In vivo experiments further confirmed that the muscle fibre diameter and muscle mass of KLF5-edited sheep were significantly increased.This study reveals the negative regulatory role of KLF5 in sheep muscle development,providing new targets for the genetic improvement of meat production traits.关键词
细胞/增殖/产肉性状/肌肉/调控Key words
cells/proliferation/meat production traits/muscle/regulation分类
农业科技引用本文复制引用
王丽娟..绵羊肌肉发育关键基因KLF5的CRISPR/Cas9编辑及对产肉性状的调控机制[J].中国饲料,2026,(17):15-22,8.基金项目
国家绒毛用羊产业技术体系(CARS-39-34) （CARS-39-34）
