中国实验动物学报2026，Vol.34Issue(8)：1093-1105,13.DOI:10.3969/j.issn.1005-4847.2026.08.001
实验用大林姬鼠封闭群生物学特性的系统研究
Systematic analysis of the biological characteristics of a closed colony of laboratory Apodemus peninsulae
摘要
Abstract
Objective To systematically analyze the biological characteristics of Apodemus peninsulae under standardized feeding conditions,including growth and development,reproductive performance,hematological and biochemical parameters,anatomical and histological characteristics,thereby providing baseline data for the standardization of this species as a laboratory animal.Methods Forty male and 40 female Apodemus peninsulae aged 4 or 8 weeks were included.Body mass and size,reproductive performance,14 hematological and 12 serum biochemical parameters,organ mass and coefficients,and skeletal bone counts were measured.Major organs were examined by hematoxylin-eosin(HE)staining,and liver tissue was evaluated using Oil Red O and Masson's trichrome staining.Results The mean neonatal mass was(2.259±0.382)g.Rapid growth occurred from 14 d of age and slowed after 58 d,with significant sexual dimorphism in body mass.The mean litter size increased from(5.20±1.65)in the F1 generation to(6.42±1.28)in the F4 generation.Multiple hematological and biochemical parameters varied by age and sex.Serum levels of alanine aminotransferase,aspartate aminotransferase,and their serum ALT,AST and TG levels were different from those reported in C57BL/6 mice.At 8 weeks of age,mild hepatic lipid deposition and inflammatory infiltration were observed,accompanied by positive Oil Red O staining.Conclusions This study provides a comprehensive characterization of the biological features of Apodemus peninsulae,establishing baseline reference data to support its standardization as a laboratory animal and the development of disease models.The findings also suggest that Apodemus peninsulae may serve as a spontaneous animal model of metabolic dysfunction-associated steatotic liver disease(MASLD).关键词
SPF级大林姬鼠/封闭群/生物学特性/生长发育/血液生理生化/脏器系数Key words
SPF grade Apodemus peninsulae/closed colony/biological characteristics/growth and development/blood physiology and biochemistry/organ coefficients分类
生物科学引用本文复制引用
何佳蕾,丁瑜,张晴,张曦文,李衍尊,高妍,袁宝,陈健,任文陟,胡进平..实验用大林姬鼠封闭群生物学特性的系统研究[J].中国实验动物学报,2026,34(8):1093-1105,13.基金项目
国家重点研发计划(2022YFF0710503),国家自然科学基金(32470568),吉林省科技发展计划项目(20260206006ZP).Funded by the National Key Research and Development(R&D)Program of China(2022YFF0710503),the National Natural Science Foundation of China(32470568),the Jilin Provincial Science and Technology Development Plan Project(20260206006ZP). （2022YFF0710503）
