中国实验动物学报2026，Vol.34Issue(8)：1120-1134,15.DOI:10.3969/j.issn.1005-4847.2026.08.003
健脾舒胰汤调控花生四烯酸代谢改善慢性胰腺炎的作用机制
Chronic pancreatitis improvement by Jianpi Shuyi decoction through arachidonic acid metabolism regulation
摘要
Abstract
Objective To investigate the mechanism of action of Jianpi Shuyi Decoction in regulating arachidonic acid(AA)metabolism in ameliorating dibutyltin dichloride(DBTC)-induced chronic pancreatitis(CP)in rats.Methods A CP model was established in male SD rats through a single tail vein injection of DBTC.Rats were randomly allocated into normal(n=8)and model groups(n=42).On day 2 post-modeling,32 surviving model rats were further randomized into model group,low-Jianpi Shuyi decoction group(L-JPSY group,3.4 g/kg),high-dose Jianpi Shuyi decoction group(H-JPSY group,17 g/kg),and coenzyme Q10(5 mg/kg)group(n=8 each).Treatments were administered once daily by gavage for 3 weeks.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),direct bilirubin(DBIL),and alkaline phosphatase(ALP)were measured using an automated biochemical analyzer,and interleukin(IL)-1β was assessed by enzyme-linked immunosorbent assay.Pancreatic histopathology was evaluated using hematoxylin-eosin(HE)and Sirius red(SR)staining.α-SMA and Col-Ⅰ mRNA and protein expression were detected by RT-qPCR,immunohistochemistry and Western Blot.AA metabolite levels in serum and pancreatic tissues were quantified by LC-MS/MS,and the mRNA expression of key AA metabolic enzymes Ptgs1,Ptgs2,Ptges,Alox5,Alox12,and Alox 15 was analyzed in pancreatic tissues by RT-qPCR.Results Rats in the model group exhibited significantly elevated ALT,AST,TBIL,DBIL,ALP,and IL-1β serum levels compared with rats in the normal group(P＜0.01).Histological examination of pancreatic tissue revealed extensive inflammatory cell infiltration and collagen deposition.α-SMA and Col-Ⅰ mRNA and protein expression were upregulated in pancreatic tissues(P＜0.01).Serum content of the AA metabolites prostaglandin D2(PGD2)and prostaglandin E2(PGE2)were significantly higher in the model group(P＜0.05),while pancreatic tissues showed increases in PGD2,5-hydroxyeicosatetraenoic acid(5-HETE),15-hydroxy eicosatetraenoic acid(15-HETE),and 11(S)-hydroxyeicosatetraenoic acid(11(S)-HETE)(P＜0.01).Ptgs1,Ptgs2,Alox5,and Alox15 mRNA expression was significantly upregulated in pancreatic tissues(P＜0.01).Serum levels of ALT,AST,TBIL,DBIL,ALP,and IL-1β were significantly decreased in the L-JPSY group,H-JPSY group and the coenzyme Q10 group compared with those in the model group(P＜0.05,P＜0.01),and pancreatic inflammation and collagen deposition were alleviated.α-SMA and Col-Ⅰ mRNA and protein expression in pancreatic tissue were significantly reduced(P＜0.05,P＜0.01).L-JPSY group and H-JPSY group significantly reduced serum PGD2 and PGE2(P＜0.05);it also led to reduced pancreatic contents of PGD2,5-HETE,15-HETE,and 11(S)-HETE(P＜0.05,P＜0.01),along with significant downregulation of Ptgs2,Alox5,and Alox15 mRNA expression in pancreatic tissues(P＜0.05,P＜0.01).Conclusions Jianpi Shuyi decoction can significantly inhibit the progression of pancreatic fibrosis in rats with DBTC-induced chronic pancreatitis,with no significant differences between high-and low-doses.Its mechanism of action may be related to the regulation of cyclooxygenase-2 expression in the arachidonic acid pathway and the expression of 5-and 15-lipoxygenase.关键词
健脾舒胰汤/慢性胰腺炎/花生四烯酸代谢/胰腺纤维化/二丁基二氯化锡Key words
Jianpi Shuyi decoction/chronic pancreatitis/arachidonic acid metabolism/pancreatic fibrosis/dibutyltin dichloride分类
生物科学引用本文复制引用
李甫,甘可欣,刘伟,陈萌,胡永红,徐东亮..健脾舒胰汤调控花生四烯酸代谢改善慢性胰腺炎的作用机制[J].中国实验动物学报,2026,34(8):1120-1134,15.基金项目
国家自然科学基金项目(82405249),上海市自然科学基金项目(24ZR1467000),上海中医药大学科技发展项目(24KFL064).Funded by National Natural Science Foundation of China(82405249),Natural Science Foundation of Shanghai(24ZR1467000),Science and Technology Development Project of Shanghai University of Traditional Chinese Medicine(24KFL064). （82405249）
