中国实验方剂学杂志2026，Vol.32Issue(19)：22-31,10.DOI:10.13422/j.cnki.syfjx.20250719
基于PPAR信号通路探讨二陈汤对肥胖型CUMS小鼠的影响及作用机制
Erchentang Alleivates Depression in Obese Mice by Regulating PPAR Signaling Pathway
摘要
Abstract
Objective:To observe the therapeutic effect of Erchentang on depression in obese mice and explore the therapeutic mechanism.Methods:C57BL/6J mice were randomized into the control,model,metformin(0.65 g·kg-1),and low-,medium-,and high-dose(3.35,6.7,and 13.4 g·kg-1,respectively)Erchentang groups.Gavage was initiated simultaneously with modeling and continued for 28 days.The therapeutic effects of Erchentang were evaluated through behavioral tests,liver and brain indices,liver function,lipid indicators,and histopathological changes in the liver and brain tissue.RNA-seq technology was used to conduct transcriptomic analysis of mouse liver tissue,and differentially expressed genes were screened and subjected to Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Key genes were further verified by Western blot and Real-time polymerase chain reaction(Real-time PCR).Results:Behavioral tests,liver function,lipid indicators,and histopathological changes in the liver and brain tissue all demonstrated that Erchentang had therapeutic effects on depression in obese mice.KEGG pathway enrichment analysis of transcriptomic data indicated that Erchentang treated depression in obese mice mainly through the peroxisome proliferator-activated receptor(PPAR)signaling pathway.Western blot and Real-time PCR results showed that compared with the model group,the low-,medium-,and high-dose Erchentang groups exhibited downregulated expression of fatty acid-binding protein(FABP)1(P<0.01)and upregulated expression of acyl-CoA oxidase(ACOX)1,PPARα,cholesterol 7α-hydroxylase(CYP7A)1,and phosphoenolpyruvate carboxy kinase(PCK)1(P<0.05)in the liver tissue.Conclusion:Erchentang exerts therapeutic effects on obesity-associated depression by regulating the PPAR signaling pathway and the expression of related genes FABP1,ACOX1,PPARα,CYP7A1,and PCK1.关键词
过氧化物酶体增殖物激活受体(PPAR)信号通路/二陈汤/肥胖型抑郁/作用机制Key words
peroxisome proliferator-activated receptor(PPAR)signaling pathway/Erchentang/obesity-associated depression/therapeutic mechanism分类
医药卫生引用本文复制引用
俞桥,胡诗维,张金融,徐俊,赵敏..基于PPAR信号通路探讨二陈汤对肥胖型CUMS小鼠的影响及作用机制[J].中国实验方剂学杂志,2026,32(19):22-31,10.基金项目
湖北省自然科学基金联合项目(2024AFD328) （2024AFD328）
