中国实验方剂学杂志2026，Vol.32Issue(19)：32-41,10.DOI:10.13422/j.cnki.syfjx.20250919
左归丸调节肠道菌群改善肾病综合征大鼠的机制
Mechanism of Zuogui Wan in Improving Nephrotic Syndrome in Rats Based on Regulating Gut Microbiota
摘要
Abstract
Objective:To investigate the protective effects of Zuogui Wan(ZGW)decoction on nephrotic syndrome(NS)through the regulation of gut microbiota.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into five groups,with 6 rats in each group:The control group,model group,low-dose group,medium-dose group,and high-dose group.The NS rat model was induced by tail vein injection of 6.5 mg·kg-1 doxorubicin.After successful modeling,the control and model groups were gavaged with normal saline(10 mL·kg-1)twice daily,while the low-(50 mg·kg-1),medium-(100 mg·kg-1),and high-dose(200 mg·kg-1)groups were gavaged with ZGW(10 mL·kg-1)twice daily.All rats were administered at fixed intervals daily for 8 consecutive weeks.The therapeutic effects of ZGW on NS in rats were observed,and the optimal dose was screened.Renal and intestinal pathological changes in rats were examined.Fecal samples were collected,and 16S rDNA sequencing technology was used to detect the diversity and abundance of gut microbiota in each group.Results:Compared with the control group,24-hour urinary protein,serum creatinine,urea nitrogen,and cholesterol levels significantly increased in the model group(P<0.01),while serum albumin levels decreased(P<0.01).These changes were ameliorated in the treatment groups(P<0.05,P<0.01).Renal pathology revealed intact and orderly arranged glomeruli and renal tubules in the control group.In the model group,glomerular atrophy,widened mesangial areas,podocyte swelling,disordered renal tubular arrangement,and numerous casts were observed.Varying degrees of glomerular atrophy and disordered renal tubular arrangement were seen in the treatment groups.Specific protein assays revealed increased serum levels of kidney injury molecule-1(KIM-1)and α1-microglobulin(P<0.01),and significantly decreased levels of E-cadherin,Podocin,and Nephrin in renal tissues(P<0.01),along with a significant increase in transforming growth factor-β(TGF-β)(P<0.01)in the model group.In the treatment groups,serum KIM-1 and α1-microglobulin levels decreased,E-cadherin levels significantly increased(P<0.01),and TGF-β levels significantly decreased(P<0.01).Podocin and Nephrin levels increased in the high-dose group(P<0.05).Additionally,intestinal mucosal atrophy,epithelial cell edema,decreased goblet cells,and multiple inflammatory infiltrations were observed in the model group.The sequencing results of gut microbiota demonstrate that ZGW can significantly ameliorate intestinal dysbiosis in rats,restore the abundance of beneficial bacteria,and exert a profound influence on the compositional profile of gut microbiota.Conclusion:ZGW can alleviate renal injury in rats with nephrotic syndrome by improving gut microbiota and protecting the intestinal barrier.关键词
左归丸/肾病综合征/肠道菌群/大鼠Key words
Zuogui Wan/nephrotic syndrome/gut microbiota/rats分类
医药卫生引用本文复制引用
胡鑫宇,张涂贝,胡一昕,王瑞,熊致立,蔡燕,张雪荣,王林群..左归丸调节肠道菌群改善肾病综合征大鼠的机制[J].中国实验方剂学杂志,2026,32(19):32-41,10.基金项目
第六批全国中医临床优秀人才研修项目(国中医药人教函[2025]256 号) （国中医药人教函[2025]256 号）
湖北省自然科学基金(联合基金)项目(2023AFD149) （联合基金）
湖北省中医药管理局2023-2024年度中医药青年人才项目(ZY2023Q003) （ZY2023Q003）
