中国兽医科学2026，Vol.56Issue(9)：1171-1177,7.DOI:10.16656/j.issn.1673-4696.2026.0152
基因Ⅰ型日本脑炎病毒亚单位疫苗的构建与免疫原性评价
Construction and immunogenicity evaluation of a genotype Ⅰ Japanese encephalitis virus subunit vaccine
摘要
Abstract
Given the urgency of Japanese encephalitis(JE)prevention and control in pig farms in China,as well as the uncertainty regarding the protective efficacy of currently available commercial genotype Ⅲ(GⅢ)Japanese encephalitis virus(JEV)vaccines against circulating genotype Ⅰ(GⅠ)JEV strains,there is an urgent need to develop safe,highly effective,and broad-spectrum novel JEV vaccines.The E protein gene of GⅠJEV was inserted into the multiple cloning sites of the baculovirus vector pFastBac Dual to construct a recombinant plasmid.The sequence-verified positive recombinant plasmid was transformed into DH10Bac competent cells,and recombinant bacmids were obtained through blue-white colony screening.After PCR verification,the recombinant bacmids were transfected into Sf9 cells to generate recombinant baculovirus rvBacmid-GⅠ-E.After successive passaging,expression and purification were performed,and the target protein was successfully identified by SDS-PAGE and Western-blot.Mouse immunization experiments demonstrated that vaccination with GⅠ-E significantly stimulated the production of JEV-specific IgG antibodies and markedly increased the levels of cytokines,including IL-2,IL-4,TNF-α,and IFN-γ.This study provides a novel immunization strategy and theoretical basis for the prevention of JE.关键词
日本脑炎病毒/杆状病毒表达载体系统/亚单位疫苗/免疫原性评价Key words
Japanese encephalitis virus/baculovirus expression vector system/subunit vaccine/immunogenicity evaluation分类
农业科技引用本文复制引用
黄晋炜,谭书桢,孙世琪,郭慧琛,白满元,郑泽中..基因Ⅰ型日本脑炎病毒亚单位疫苗的构建与免疫原性评价[J].中国兽医科学,2026,56(9):1171-1177,7.基金项目
甘肃省技术创新引导计划－东西部科技协作专项(24CXNA030) （24CXNA030）
甘肃省省级人才项目－青年团队项目(2025QNTD34) （2025QNTD34）
甘肃省重点研发计划项目(25YFNA014) （25YFNA014）
兰州市青年科技人才创新项目(2024-QN-3) （2024-QN-3）
宁夏重点研发计划项目(2024BBF02017) （2024BBF02017）
