中国兽医科学2026，Vol.56Issue(9)：1178-1187,10.DOI:10.16656/j.issn.1673-4696.2026.0135
O型口蹄疫病毒非结构蛋白2C突变毒株的构建及其生物学特性分析
Construction of a serotype O foot-and-mouth disease virus nonstructural protein 2C mutant and analysis of its biological characteristics
摘要
Abstract
To elucidate how the T135I substitution in the nonstructural protein 2C affects the ca-pacity of foot-and-mouth disease virus(FMDV)serotype O to survive intracellularly during the estab-lishment of persistent infection,a recombinant FMDV harboring the T135I mutation in the 2C protein was constructed using reverse genetics and its replicative fitness was analyzed through growth curve analysis,real-time quantitative PCR,and Western-blotting for phenotypic characterization.The mutant was successfully rescued and remained genetically stable.It exhibited significantly higher viral titres,as well as increased genomic and protein expression,in BHK-21 cells compared with the parental strain(P<0.05),whereas these parameters were markedly reduced in MDBK cells(P<0.05).Collective-ly,these data identify residue 2C-T135 as a critical molecular determinant of FMDV adaptation to the MDBK cellular environment and highlight the context-dependent functionality of this locus.The gener-ated mutant provides a robust platform for further dissecting FMDV persistent infection mechanisms and virus-host adaptive interplay.关键词
口蹄疫病毒/持续性感染/反向遗传学技术/点突变/生物学特性Key words
foot-and-mouth disease virus/persistent infection/reverse genetics/point mutation/biological characteristics分类
农业科技引用本文复制引用
赵丽容,王爽,郭慧琛,孙世琪,马全英,王晓强..O型口蹄疫病毒非结构蛋白2C突变毒株的构建及其生物学特性分析[J].中国兽医科学,2026,56(9):1178-1187,10.基金项目
国家重点研发计划项目(2023YFD1800304) （2023YFD1800304）
甘肃省人才项目(青年团队)(2025QNTD34) （青年团队）
甘肃省青年科技基金项目(25JRRA442) （25JRRA442）
甘肃省科技厅自然科学基金项目(25JRRA176) （25JRRA176）
