中国兽医科学2026，Vol.56Issue(9)：1195-1201,7.DOI:10.16656/j.issn.1673-4696.2026.0120
表达尼帕病毒F和G囊膜糖蛋白的VSV假病毒的构建与优化
Construction and optimization of a recombinant vesicular stomatitis virus pseudovirus expressing Nipah virus F and G envelope glycoproteins
摘要
Abstract
To enable safe and effective Nipah virus(NiV)studies under lower biosafety conditions,this research aimed to construct a pseudovirus model that carried a reporter gene and mimics the NiV invasion process.This study utilized the established rVSV-ΔG-EGFP pseudovirus system based on vesicular stomati-tis virus(VSV),where the VSV G glycoprotein gene was replaced with enhanced green fluorescent protein(EGFP)in the viral genome.Following exogenous expression of NiV F and G proteins in cells,cells were transfected with the rVSV-ΔG-EGFP backbone to package the recombinant pseudovirus rVSV-ΔG-NiV F/G-EGFP.Transmission electron microscopy and Western-blot analysis confirmed successful packaging of the pseu-dovirus particles and correct expression of the envelope proteins.Neutralization assays demonstrated that this pseudovirus could be effectively inhibited by NiV-specific neutralizing antibodies.The con-structed pseudovirus system provides a safe and effective technical platform for evaluating NiV neutral-izing antibodies,screening therapeutic candidates,and investigating viral entry mechanisms.关键词
尼帕病毒/假病毒/水疱性口炎病毒/囊膜蛋白Key words
Nipah virus/pseudovirus/vesicular stomatitis virus/viral envelope proteins分类
农业科技引用本文复制引用
邝瑞欣,宋翠萍,丁铲,孙英杰,徐瑞娟,田雨菲,刘彦峰,赵飞杨,屈阳,廖瑛,仇旭升,谭磊..表达尼帕病毒F和G囊膜糖蛋白的VSV假病毒的构建与优化[J].中国兽医科学,2026,56(9):1195-1201,7.基金项目
中国农业科学院科学中心项目(CAAS-CSLPDCP-202402) （CAAS-CSLPDCP-202402）
国家重点研发计划项目(2022YFD1801500) （2022YFD1801500）
