中国兽医杂志2026，Vol.62Issue(9)：47-54,8.DOI:10.20157/j.cnki.zgsyzz.2026.sf.061
原花青素A1抗猪流行性腹泻病毒作用及其对细胞自噬和线粒体功能的影响
Antiviral Activity of Procyanidin A1 against Porcine Epidemic Diarrhea Virus and Its Effect on Cellular Autophagy and Mitochondrial Function
摘要
Abstract
To investigate the effects of porcine epidemic diarrhea virus(PEDV)infection on cellular autophagy,mitochondrial dynamics,and mitophagy,and to evaluate the antiviral activity of procyanidin A1,this study examined the autophagy-related proteins microtubule-associated protein 1A/1B light chain 3-Ⅱ(LC3-Ⅱ)and ubiquitin-binding protein p62(p62),mitochondrial dynamics-related proteins mitofusin 1(MFN1)and dynamin-related protein 1(DRP1),and mitophagy-related proteins PTEN-induced putative kinase 1(PINK1)and Parkin RBR E3 ubiquitin protein ligase(Parkin)after PEDV infection and procyanidin A1 treatment using cell viability assays,Western blot(WB),indirect immunofluorescence assay(IFA),and mCherry-GFP-LC3 dual-fluorescence plasmid transfection.The results showed that procyanidin A1 treatment at concentrations below 50 μg/mL had no significant effect on the viability of Vero cells.Compared with the Mock control group,PEDV infection significantly increased LC3-Ⅱ protein expression(P<0.001),decreased p62 protein expression(P<0.05 or P<0.001),and promoted autophagic flux.PEDV infection also significantly increased DRP1 protein expression(P<0.01 or P<0.001),decreased MFN1 protein expression(P<0.05 or P<0.001),and upregulated the expression of PINK1(P<0.05)and Parkin(P<0.01 or P<0.001).After treatment with 30 μg/mL procyanidin A1,viral expression was significantly reduced compared with the Mock control group(P<0.001).Compared with the PEDV infection group,p62 protein expression was restored(P<0.01),LC3-Ⅱ protein expression was significantly downregulated(P<0.01),which was consistent with the results of the mCherry-GFP-LC3 dual-fluorescence plasmid transfection assay.In addition,DRP1 protein expression was significantly decreased(P<0.001),and Parkin protein expression was significantly reduced(P<0.01).These results indicate that PEDV infection affects autophagy-related proteins,mitochondrial dynamics-related proteins,and mitophagy-related proteins,whereas procyanidin A1 treatment reverses these alterations.This study provides new insights into potential therapeutic targets for PEDV infection and the molecular mechanisms underlying the antiviral activity of procyanidin.关键词
猪流行性腹泻病毒(PEDV)/原花青素/细胞自噬/线粒体膜动力学/线粒体自噬/抗病毒Key words
porcine epidemic diarrhea virus(PEDV)/procyanidin/autophagy/mitochondrial dynamics/mitophagy/antiviral分类
农业科技引用本文复制引用
翁与竞,李嘉琳,曹宗喜,王晓佳,王九峰..原花青素A1抗猪流行性腹泻病毒作用及其对细胞自噬和线粒体功能的影响[J].中国兽医杂志,2026,62(9):47-54,8.基金项目
国家重点研发计划(2023YFD1801100) （2023YFD1801100）
海南省科技专项(ZDYF2024XDNY198) （ZDYF2024XDNY198）
