中国兽医杂志2026，Vol.62Issue(9)：72-77,6.DOI:10.20157/j.cnki.zgsyzz.2026.sf.068
非洲猪瘟病毒E248R蛋白的表达和免疫原性评价
Expression and Immunogenicity Evaluation of African Swine Fever Virus E248R Protein
摘要
Abstract
To prepare recombinant African swine fever virus(ASFV)E248R protein using the baculovirus expression system and evaluate its immunogenicity,an E248R recombinant baculovirus expression vector was constructed.The target protein was expressed and purified in Spodoptera frugiperda Sf9 cells,and its ability to induce humoral and cellular immune responses was evaluated in mice.The results showed that the recombinant ASFV E248R protein was efficiently expressed in Sf9 cells and specifically recognized by ASFV-positive serum.Animal immunization experiments demonstrated that the recombinant ASFV E248R protein induced high levels of specific immunoglobulin G(IgG)antibodies in mice.The cell proliferation assay(CCK-8)showed that the stimulation index(SI)of splenic lymphocytes in the ASFV E248R recombinant protein-immunized group was significantly higher than that in the PBS control group(P<0.000 1).Enzyme-linked immunospot assay(ELISpot)revealed that the recombinant ASFV E248R protein significantly promoted interferon-γ(IFN-γ)secretion by lymphocytes(P<0.001).Flow cytometry analysis showed that the recombinant ASFV E248R protein significantly increased the proportions of CD4⁺(P<0.000 1)and CD8⁺ T lymphocytes(P<0.01).These results indicate that the ASFV E248R recombinant protein was successfully prepared and could induce strong humoral and cellular immune responses in mice,suggesting its potential as a candidate antigen for ASFV subunit vaccines.关键词
非洲猪瘟病毒(ASFV)/E248R蛋白/昆虫杆状病毒表达系统/免疫原性/亚单位疫苗Key words
African swine fever virus(ASFV)/E248R protein/baculovirus-insect cell expression system/immunogenicity/subunit vaccine分类
农业科技引用本文复制引用
王士源,刘畅,谢振华,李劼,刘英楠,陈鸿军,苗晋锋..非洲猪瘟病毒E248R蛋白的表达和免疫原性评价[J].中国兽医杂志,2026,62(9):72-77,6.基金项目
国家自然科学基金(32570161) （32570161）
国家重点研发计划(2025YFD1800600) （2025YFD1800600）
