中国药房2026，Vol.37Issue(17)：2254-2257,4.DOI:10.6039/j.issn.1001-0408.2026.17.07
通关藤饮片中两种特异性C21甾体皂苷含量测定方法的建立
Establishment of content determination method for two specific C21-steroid saponins in Marsdenia tenacissima decoction pieces
摘要
Abstract
OBJECTIVE To establish a content determination method for two specific C21-steroid saponins namely 3-O-β-allopyranosyl-(1→4)-β-oleandropyranosyl-11-O-isobutyryl-12-O-acetyl tenacigenin B(compound T)and 17β-tenacigenin B in Marsdenia tenacissima decoction pieces.METHODS Ultra-high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was adopted.An XSelect HSS T3 XP chromatographic column was used.Gradient elution was performed with 0.1%formic acid aqueous solution-methanol as the mobile phase.The flow rate was 0.3 mL/min,the injection volume was 5 μL,and the column temperature was 40℃.An electrospray ionization source was applied for mass spectrometry,and multiple reaction monitoring mode was used for positive-and negative-ion acquisition.RESULTS The linear relationships of compound T and 17β-tenacigenin B were good within the concentration ranges of 10-200 ng/mL and 20-200 ng/mL,with r values of 0.993 1 and 0.998 6,respectively;the RSD values of precision,repeatability,accuracy,and stability were all below 4.5%(n=5-6).Their contents in three batches of decoction pieces were 94.0-102.8 μg/g and 1 705.4-1 736.2 μg/g,respectively.CONCLUSIONS The established method can be applied for the content determination of the two specific C21-steroid saponins mentioned above in M.tenacissima decoction pieces.关键词
通关藤/11-O-异丁酰基-12-O-乙酰基通关藤苷元B-3-O-茯苓二糖基/17β-通关藤苷元B/含量测定Key words
Marsdenia tenacissima/3-O-β-allopyranosyl-(1→4)-β-oleandropyranosyl-11-O-isobutyryl-12-O-acetyl tenacigenin B/17β-tenacigenin B/content determination分类
医药卫生引用本文复制引用
王祁民,鞠倩,范理菊,李颖,马银玲,董占军..通关藤饮片中两种特异性C21甾体皂苷含量测定方法的建立[J].中国药房,2026,37(17):2254-2257,4.基金项目
国家自然科学基金项目(No.82104286) （No.82104286）
河北省自然科学基金项目(No.H2020307043) （No.H2020307043）
河北省医学科学研究课题(No.20230353) （No.20230353）
