中国药房2026，Vol.37Issue(17)：2258-2263,6.DOI:10.6039/j.issn.1001-0408.2026.17.08
不同砂炒时间钩吻炮制品的颜色、成分、抗肿瘤活性变化及相关性研究
Study on changes and correlations of color,components and anti-tumor activity of processed products of Gelsemium elegans at different sand-frying durations
摘要
Abstract
OBJECTIVE To explore the changes and correlations of color,components and anti-tumor activity of Gelsemium elegans during sand-frying processing,so as to provide a reference for the toxicity-attenuating and efficacy-preserving processing as well as quality control of sand-fried G.elegans decoction pieces.METHODS Raw G.elegans and its processed products prepared by sand-frying for 15,30,75,105 and 120 s were taken as test samples.The chromatic values,contents of total alkaloids and seven main active components(gelsemine,humantenine,koumine,gelsenicine,etc.)were determined.Their in vitro anti-tumor activity was investigated.The correlations between color and component contents,as well as between total alkaloid content and anti-tumor activity were analyzed.RESULTS The color of G.elegans samples generally darkened after sand-frying,and the total color difference(ΔE*)of samples sand-fried for 75 s was greater than 20.At the initial stage of sand-frying(15-30 s),the total alkaloid content in processed products decreased sharply compared with the raw material;at the later-stage of sand-frying(75-105 s),the declining rate of total alkaloid content slowed down and tended to be stable.The contents of gelsenicine and humantenidine in processed products dropped drastically at 30 s of sand-frying,while the contents of koumine and sempervirine decreased substantially(by about 80%)after 75 s of sand-frying and then gradually tended to be stable.The anti-HepG2 cell proliferation activity of each processed product decreased with the extension of frying time.Correlation analysis demonstrated that color brightness and yellow-blue chromaticity were significantly positively correlated with the contents of total alkaloids and all determined active components except gelsemine(P＜0.05 or P＜0.01);red-green chromaticity was significantly positively correlated with the contents of koumine,sempervirine,gelsemivirine and humantenidine(P＜0.05 or P＜0.01);ΔE* was significantly negatively correlated with the contents of total alkaloids and all determined active components except gelsemine.Besides,total alkaloid content was significantly negatively correlated with the half maximal inhibitory concentration of the samples on HepG2 cells(P＜0.05 or P＜0.01).CONCLUSIONS With the prolongation of sand-frying time,the color of sand-fried G.elegans decoction pieces gradually turned brownish-brown,the contents of seven active components and total alkaloids decreased progressively,and its anti-HepG2 cell activity showed an overall downward trend.Sand-frying for 75 s serves as a critical turning-point for the above-mentioned changes.关键词
钩吻/砂炒钩吻/色度值/活性成分/抗肿瘤活性/总生物碱/相关性分析/减毒存效Key words
Gelsemium elegans/sand-fried Gelsemium elegans/chromatic value/active components/anti-tumor activity/total alkaloids/correlation analysis/toxicity-attenuating and efficacy-preserving分类
医药卫生引用本文复制引用
吴昊,陈莹,陈丽松,黄成龙,王月灵,陈丽蓉,王文义,王英豪,李德森,吴水生..不同砂炒时间钩吻炮制品的颜色、成分、抗肿瘤活性变化及相关性研究[J].中国药房,2026,37(17):2258-2263,6.基金项目
国家中医药管理局高水平中医药重点学科建设项目-临床中药学(No.zyyzdxk-2023107) （No.zyyzdxk-2023107）
国家中医药管理局全国老药工传承工作室建设项目(No.国中医药人教函[2025]181号) （No.国中医药人教函[2025]181号）
福建省科技计划引导性项目(No.2025Y0027) （No.2025Y0027）
福建中医药大学高层次人才科研项目(No.X2024010-人才) （No.X2024010-人才）
