中国药业2026，Vol.35Issue(18)：38-43,6.DOI:10.3969/j.issn.1006-4931.2026.18.009
大戟醇对MH7A细胞凋亡、迁移及侵袭的影响
Effects of Euphol on Apoptosis,Migration and Invasion of MH7A Cells
摘要
Abstract
Objective To investigate the effects of euphol on apoptosis,migration and invasion of rheumatoid arthritis(RA)fibroblast-like synoviocytes MH7A cells.Methods MH7A cells were treated with euphol at concentrations of 0(blank control),3.125,6.25,12.5,25,50,100 μg/mL and methotrexate(MTX)at 20 μg/mL for 12,24 and 48 h.Cell viability was detected by CCK-8 method.The cells were divided into the blank control group,MTX group(20.0 μg/mL),and euphol low-,medium-and high-dose groups(25,50,100 μg/mL),and all cells were cultured in complete MH7A culture medium for 24 h.Flow cytometry was used to detect cell apoptosis,wound healing assay was used to detect cell migration ability,Transwell invasion assay was used to detect cell invasion ability,and enzyme-linked immunosorbent assay was used to detect the expression levels of matrix metalloproteinase-3(MMP-3),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in cell supernatant.Results Compared with the blank control,cell viability was significantly decreased after treatment with MTX and euphol at 50 and 100 μg/mL for 12,24 and 48 h(P<0.05).Compared with the blank control group,the apoptosis rates in the MTX group and euphol high-dose group were significantly increased,while the cell migration index and the expression level of TNF-α were significantly decreased(P<0.05);the cell invasion indexes in the MTX group and euphol medium-and high-dose groups,and the expression levels of MMP-3,IL-6 and IL-1β were significantly decreased(P<0.05).Conclusion Euphol can exert anti-RA effects by inhibiting MH7A cell viability,promoting cell apoptosis,and attenuating cell migration and invasion abilities.关键词
大戟醇/MH7A细胞/凋亡/迁移/侵袭Key words
euphol/MH7A cells/apoptosis/migration/invasion分类
医药卫生引用本文复制引用
刘霞,李澳文,李晨阳,陈燕,赵军..大戟醇对MH7A细胞凋亡、迁移及侵袭的影响[J].中国药业,2026,35(18):38-43,6.基金项目
国家自然科学基金[82360764]. （）
