中国中药杂志2026，Vol.51Issue(18)：5198-5207,10.DOI:10.19540/j.cnki.cjcmm.20260523.801
疏风解毒胶囊对甲型H1N1流感病毒肺炎小鼠恢复期肺损伤修复及T细胞亚群比例的影响
Effects of Shufeng Jiedu Capsules on lung injury repair and T cell subset proportions during recovery period in mice with influenza A H1N1 pneumonia
摘要
Abstract
This study investigated the effects of Shufeng Jiedu Capsules(SFJD)on pharmacodynamics,T helper cell(Th)proportions,and CD8+T-cell immune memory formation during the recovery phase in mice infected with influenza A H1N1 virus.A non-lethal pneumonia mouse model was established by intranasal inoculation with influenza A virus A/PR/8/34(PR8)strain.Mice were randomly divided into three time-point groups:7,10,and 13 days post-infection(dpi),with 30 mice in each group.At each time point,mice were further randomly divided into a normal group,PR8 model group,positive drug group(oseltamivir phosphate,0.027 5 g·kg-1·d-1),and high-and low-dose SFJD groups(2.558 and 1.279 g·kg-1·d-1),with six mice in each subgroup.From 0 dpi until the day of sample collection(7,10,or 13 dpi),mice were administered the corresponding drugs by gavage,while the normal and PR8 model groups received equal volumes of deionized water.At the indicated time points,mice were sacrificed for sample collection.Lung and body weights were measured to calculate the lung index.At 10 dpi,Micro-CT was used to observe lung imaging changes.Viral load in lung tissue was detected by qRT-PCR.Flow cytometry was used to determine the proportions of CD4+T cells and CD8+T cells in peripheral blood and lung tissue at the three time points.Based on preliminary experimental results,10 dpi was selected for further analysis of T-cell subsets in lung tissue by flow cytometry,including Th1 and Th2,as well as naive CD8+T cells,central memory CD8+T cells,effector CD8+T cells,and memory precursor effector CD8+T cells.The results showed that SFJD increased body weight and reduced lung index at all three time points,improved lung morphology at 10 dpi,and reduced viral load in lung tissue.SFJD also increased the proportion of CD8+T cells in peripheral blood at 7 dpi,increased the proportion of CD4+T cells in lung tissue at 7 dpi,and reduced the proportion of CD8+T cells in lung tissue at 7,10,and 13 dpi.Based on analyses of lung index and the proportions of CD4+and CD8+T cells in peripheral blood and lung tissue at the three time points,10 dpi was selected to investigate the effects of SFJD on T-cell subsets in lung tissue.SFJD promoted recovery from mild influenza A H1N1 viral pneumonia by inhibiting differentiation of Th1 cells and effector CD8+T cells,while promoting the generation of naive CD8+T cells,central memory CD8+T cells,and memory precursor effector CD8+T cells,thereby preventing persistent inflammation and facilitating immune memory formation.关键词
甲型H1N1流感病毒/病毒性肺炎/疏风解毒胶囊/细胞免疫/T细胞亚群Key words
influenza A virus H1N1/viral pneumonia/Shufeng Jiedu Capsules/cellular immunity/T cell subsets引用本文复制引用
李欣颖,包蕾,李舒冉,赵荣华,孙静,谢丹,郭姗姗,崔晓兰,耿子涵..疏风解毒胶囊对甲型H1N1流感病毒肺炎小鼠恢复期肺损伤修复及T细胞亚群比例的影响[J].中国中药杂志,2026,51(18):5198-5207,10.基金项目
国家自然科学基金项目(82305078) （82305078）
中国中医科学院科技创新工程项目(CI2021A04620) （CI2021A04620）
中医疫病防治药效评价创新体系构建及新药研发项目(ZXKT25038) （ZXKT25038）
