中国中药杂志2026，Vol.51Issue(18)：5208-5215,8.DOI:10.19540/j.cnki.cjcmm.20260427.401
热感赛比斯坦颗粒调控MAPK/NF-κB通路改善哮喘小鼠气道炎症反应
Regan Saibisitan Granules alleviate airway inflammatory responses in asthmatic mice by regulating MAPK/NF-κB pathway
摘要
Abstract
The aim of this paper was to explore the effect and mechanism of Regan Saibisitan Granules(RGS)on improving airway inflammatory responses in asthmatic mice by regulating the mitogen-activated protein kinase(MAPK)/nuclear factor-κB(NF-κB)signaling pathway.Female Balb/c mice were randomly divided into a blank group,a model group,low-,medium-and high-dose RGS groups(0.75,1.5,and 3 g·kg-1),dexamethasone group(3 mg·kg-1),and a Feining Tablets group(0.72 g·kg-1)according to body weight.The asthma model was established by ovalbumin(OVA)sensitization combined with aerosol challenge.The numbers of white blood cells(WBC),eosinophils(EOS),neutrophils(NEU),and lymphocytes(LYM)in bronchoalveolar lavage fluid(BALF)were counted.The levels of interleukin-4(IL-4),interleukin-5(IL-5),interleukin-13(IL-13),interferon-γ(INF-γ)in BALF,as well as the secretion of immunoglobulin E(IgE),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)in serum,were detected using enzyme-linked immunosorbent assay(ELISA)kits.The pathological changes of lung tissue were observed by hematoxylin-eosin and periodic acid-Schiff staining to comprehensively evaluate the ameliorative effect of RGS on inflammation in asthmatic mice.The protein expression levels of phosphorylated extracellular signal-regulated kinase 1/2(p-ERK1/2),phosphorylated c-Jun N-terminal kinase(p-JNK),phosphorylated mitogen-activated protein kinase(p-p38),phosphorylated NF-κB(p-NF-κB),phosphorylated inhibitor of NF-κB α(p-IκBα),cleaved caspase-3 and B-cell lymphoma/leukemia-2-associated X protein(Bax)in lung tissue were detected by Western blot.The results showed that compared with the model group,the numbers of WBC,EOS,NEU and LYM in BALF of the RGS groups,the Feining Tablets group and the dexamethasone group decreased,and the content of IL-4,IL-5,IL-13 in BALF as well as the levels of IgE,TNF-α and IL-1β in serum significantly decreased.In addition,inflammatory cell infiltration,airway wall thickening,and mucus secretion were significantly alleviated,and the levels of p-ERK1/2,p-JNK,p-p38,p-NF-κB,p-IκBα,as well as the protein levels of cleaved caspase-3 and Bax in lung tissue of the RGS groups decreased.In conclusion,RGS may inhibit inflammatory response and cell apoptosis by regulating the MAPK/NF-κB signaling pathway,thereby alleviating airway inflammation in asthmatic mice.关键词
哮喘/热感赛比斯坦颗粒/MAPK/NF-κB通路/气道炎症Key words
asthma/Regan Saibisitan Granules/MAPK/NF-κB pathway/airway inflammation引用本文复制引用
高慧敏,王玥,林明宝,徐磊,苏文灵,邢建国,郑瑞芳..热感赛比斯坦颗粒调控MAPK/NF-κB通路改善哮喘小鼠气道炎症反应[J].中国中药杂志,2026,51(18):5208-5215,8.基金项目
新疆维吾尔自治区天山英才-科技创新领军人才项目(2023TSYCLJ0042) （2023TSYCLJ0042）
新疆维吾尔自治区重大科技专项(2022294474-4-2,2022A03017-2-4) （2022294474-4-2,2022A03017-2-4）
新疆维吾尔自治区公益性科研院所基本科研业务费项目(KY2024122) （KY2024122）
中国医学科学院中央级公益性科研院所基本科研业务费专项(2024-RW350-01) （2024-RW350-01）
