中国茶叶2026，Vol.48Issue(9)：37-44,8.
文县地方茶树资源的生化成分检测、群体结构分析与基因资源的GWAS挖掘
Biochemical Profiling,Population Structure,and GWAS-Based Identification of Candidate Genes in Local Tea Germplasm from Wenxian County
摘要
Abstract
To investigate the biochemical composition characteristics,genetic background,and quality-related candi-date genes of local tea germplasm resources from Wenxian County,Gansu Province,this study systematically col-lected 150 representative tea individuals from Shuihaoping and Liziba Villages in Bikou Town,as well as Guanzi-gou Village in Fanba Town.The contents of water extract,tea polyphenols,free amino acids,and caffeine were de-termined in all samples.Meanwhile,30 representative accessions were genotyped using the tea 40K mSNP liquid-phase functional chip,and candidate genes associated with characteristic metabolic components were identified through genome-wide association study(GWAS).The results show significant differences in the mean contents of biochemical components among the tea resources from the three regions.Among them,samples from Guanzigou Village exhibited the highest mean values of tea polyphenols(22.62%),caffeine(3.69%),and the polyphenol-to-amino acid ratio(14.51),while showing the lowest mean free amino acid content(1.74%).In contrast,samples from Shuihaoping Village and Liziba Village displayed higher free amino acid contents and lower polyphenol-to-amino acid ratios.Principal component analysis(PCA)further confirmed that the biochemical characteristics of the population-type germplasms from Guanzigou Village were significantly distinct from those of the other two produc-ing areas.Genetic analysis based on the 40K mSNP chip reveals that germplasms from Shuihaoping and Guanzigou shared a closer genetic relationship,which was inconsistent with the clustering pattern based on biochemical com-ponents and suggests that environmental factors may play an important regulatory role in biochemical phenotypes.Through GWAS,a candidate gene,UBC36,encoding ubiquitin-conjugating enzyme E2 36,was identified to be sig-nificantly associated with free amino acid content,potentially participating in amino acid metabolism regulation via the ubiquitination pathway.This study provided a theoretical basis for quality evaluation,suitability determination for tea processing,and molecular marker-assisted breeding of tea germplasm resources in Wenxian County.关键词
茶树/文县/种质资源/生化成分/40K mSNP芯片/全基因组关联分析Key words
tea plant/Wen County/germplasm resources/biochemical components/40K mSNP chip/genome-wide association study分类
农业科技引用本文复制引用
马世福,梁超,李其昀,郭淑文,杨伟,梅飘,丁诗琦,郑梦霞,章志芳..文县地方茶树资源的生化成分检测、群体结构分析与基因资源的GWAS挖掘[J].中国茶叶,2026,48(9):37-44,8.基金项目
甘肃省2024年度强科技奖补资金项目(2024CX002) （2024CX002）
