中国畜禽种业2026，Vol.22Issue(9)：37-46,10.DOI:10.19543/j.cnki.1673-4556.20260203.001
家畜诱导性多能干细胞培养体系研究进展
Research progress on culture systems of induced pluripotent stem cells in livestock
摘要
Abstract
The establishment of induced pluripotent stem cells(iPSCs)represents a revolutionary breakthrough in stem cell research.After years of development,compared to the mature reprogramming techniques and culture methods for human and murine iPSCs,livestock iPSCs-while capable of sustaining proliferation in vitro and possessing limited differentiation potential-have yet to achieve in vivo pluripotency gold standards such as germline transmission.Furthermore,critical aspects including culture systems,definitive marker characterization,and differentiation efficiency remain significantly underdeveloped.This review outlines the initial materials and methods used in livestock iPSC reprogramming,discusses the applications and efficiency variations of different somatic cell types in generating livestock iPSCs,and evaluates the advantages and limitations of various delivery vectors as well as the roles of reprogramming factors in safety and efficiency.It also systematically examines how culture conditions:including feeder cells,basal media,small molecule compounds,and environmental factors—affect the stability of livestock iPSCs.Finally,the potential applications of livestock iPSCs in breeding,biomedical research,and cell-cultured meat production are explored,providing insights and directions for future advancements in this field.关键词
诱导性多能干细胞/重编程因子/小分子化合物Key words
Induced pluripotent stem cells/Reprogramming factor/Small molecule compounds分类
农业科技引用本文复制引用
李姝慧,赵文轩,高晨,高雪..家畜诱导性多能干细胞培养体系研究进展[J].中国畜禽种业,2026,22(9):37-46,10.基金项目
呼和浩特科技创新人才项目(2022RC-IRI-5) （2022RC-IRI-5）
国家重点研发计划(2022YFD1601203) （2022YFD1601203）
科技创新2030-重大项目(2023ZD0404801). （2023ZD0404801）
