种子2026，Vol.45Issue(8)：43-53,77,12.DOI:10.16590/j.cnki.1001-4705.2026.08.043
外源独脚金内酯缓解蒙古黄芪干旱胁迫的生理与转录响应
Physiological and Transcriptomic Responses of Exogenous Strigolactone Alleviating Drought Stress in Astragalus membranaceus var.mongholicus
摘要
Abstract
To investigate the physiological and molecular mechanisms by which exogenous strigolactone(SL)alleviates drought stress in Astragalus membranaceus var.mongholicus,a pot experiment with controlled watering was conducted using Astragalus membranaceus var.mongholicus as the material.4 treatments were established:normal water supply(control),drought stress,drought stress+foliar application of 0.5 μmol/L SL,and drought stress+foliar application of 5 μmol/L SL.Physiological in-dicator measurements combined with RNA-Seq transcriptome sequencing were employed to systemat-ically analyze the effects of exogenous SL on the physiological characteristics and gene expression reg-ulation of Astragalus membranaceus var.mongholicus under drought stress.The results showed that drought stress significantly inhibited the growth and photosynthesis of Astragalus membranaceus var.mongholicus and aggravated membrane lipid peroxidation damage.Exogenous SL effectively alleviated the damage caused by drought in Astragalus membranaceus var.mongholicus.Specifically,the 0.5μmol/L SL treatment mainly induced the activation of WRKY and bHLH transcription factors,syn-ergistically promoting α-linolenic acid metabolism and flavonoid biosynthesis,thereby strengthening the antioxidant defense system of the plants.In contrast,the 5 μmol/L SL treatment significantly upregu-lated the expression of MYB-related transcription factors,promoted the repair of photosynthesis-antenna pro-tein-related pathways and enhanced glutathione metabolism,and synergistically increased polysaccharide accu-mulation,thereby facilitating the transition of plants from stress defense to growth recovery.In conclusion,exogenous SL could mediate specific transcription factor regulatory networks to activate either the antioxidant defense system or photosynthetic system repair pathways,constructing a multi-dimensional response mecha-nism of Astragalus membranaceus var.mongholicus in response to drought stress.关键词
蒙古黄芪/干旱胁迫/独脚金内酯/转录组/生理机制Key words
Astragalus membranaceus var.mongholicus/drought stress/strigolactone/transcriptome/physiological mechanism分类
生物科学引用本文复制引用
孟勐,翟晓婷,张慧宇,刘乙莹,张久璇,曹阳..外源独脚金内酯缓解蒙古黄芪干旱胁迫的生理与转录响应[J].种子,2026,45(8):43-53,77,12.基金项目
河北北方学院省属高校基本科研业务费自然科学项目(JYT2025019) （JYT2025019）
