种子2026，Vol.45Issue(8)：54-59,6.DOI:10.16590/j.cnki.1001-4705.2026.08.054
基于转录组测序的金银花抗白粉病分子机制研究
Research on Molecular Mechanism of Lonicera japonica Resistance to Powdery Mildew based on Transcriptome Sequencing
摘要
Abstract
To explore the molecular mechanism of Lonicera japonica response to powdery mildew,using the new strain Zhengyinhua No.6 as the test material,a high-throughput transcriptome sequen-cing method was employed to study the changes in differentially expressed genes and metabolic path-ways in Lonicera japonica leaves after infection by powdery mildew.The results showed that there were 1 225 differentially expressed genes,among which 1 124 were up-regulated and 101 were down-regulated.KEGG analysis revealed that the significantly enriched up-regulated metabolic pathways in-cluded plant hormone signaling,plant pathogen interaction,phenylpropanoid metabolism,cuticle,periderm layer,and wax biosynthesis,etc.For the 6 candidate genes-ethylene receptor(Lj2P1103T21),brassinolide-amide synthase(Lj9A591T86),ATPase(Lj2A1239T190),fatty ac-yl-CoA reductase(Lj2A985G11),glycosyl hydrolase family(Lj6C57T5),and phenylalanine ammo-nia-lyase(Lj3A983T60)-PCR verification was conducted,and the gene expression trends were con-sistent with the PCR verification results.关键词
金银花/白粉病/转录组分析/分子机制Key words
Lonicera japonica/powdery mildew/transcriptome analysis/molecular mechanism分类
农业科技引用本文复制引用
张晓申,吴小波,田芳,申潇潇,曹辉,左红娟,申瑞楠..基于转录组测序的金银花抗白粉病分子机制研究[J].种子,2026,45(8):54-59,6.基金项目
河南省科技兴林项目"金银花新品种选育及提质增效栽培技术研究与示范"(豫财环资[2025]44号) （豫财环资[2025]44号）
