转化医学杂志2026，Vol.15Issue(10)：1658-1665,8.DOI:10.3639/j.issn.2095-3097.2026.10.005
女性T2DM合并CHD患者血清外泌体miR-21表达情况及其与血清IL-6、维生素D水平的关系
Expression of Serum Exosomal miR-21 and Its Correlations with Serum Interleukin-6 and Vitamin D Levels in Female Patients with Type 2 Diabetes Mellitus Complicated with Coronary Atherosclerotic Heart Disease
摘要
Abstract
Objective To investigate the expression of miR-21 in serum exosomes and its relationship with serum interleukin-6(IL-6)and vitamin D levels in female patients with type 2 diabetes mellitus(T2DM)complicated with coronary heart disease(CHD).Methods The clinical data of 280 female patients with T2DM admitted to the Third Hospital of Shijiazhuang from January 2023 to October 2024 were retrospectively analyzed.Patients were divided into the T2DM group(n=185)and the T2DM complicated with CHD group(n=95)according to whether they were complicated with CHD.In addition,healthy women who underwent physical examination in the Third Hospital of Shijiazhuang during the same period were selected as the control group(n=120).According to the presence or absence of adverse cardiovascular events during the 1-year follow-up period after discharge,female patients with T2DM complicated with CHD were divided into the good prognosis group(n=70)and the poor prognosis group(n=25).The general data,laboratory indicators and drug use status were compared among groups.Multivariate logistic regression analysis was performed to identify the independent risk factors and protective factors for T2DM complicated with CHD.Pearson correlation analysis was used to explore the correlation between serum miR-21 and IL-6,high-sensitivity C-reactive protein(hs-CRP),vitamin D and IL-10.ROC curves were drawn to evaluate the predictive efficacy of miR-21,IL-6,hs-CRP,vitamin D and IL-10 for the prognosis of patients with T2DM complicated with CHD.Results Multivariate Logistic regression analysis revealed that elevated body mass index,mean systolic blood pressure,mean diastolic blood pressure,exosomal miR-21,IL-6,hs-CRP,glycated hemoglobin A1c(HbA1c),low-density lipoprotein cholesterol(LDL-C)and serum creatinine(SCr)were risk factors for female patients T2DM complicated with CHD(P<0.05),while higher IL-10,vitamin D,vitamin C,high-density lipoprotein cholesterol(HDL-C)levels and use of statins,aspirin,angiotensin-converting enzyme inhibitor and/or angiotensinⅡ receptor blocker were protective factors for female patients T2DM complicated with CHD(P<0.05).Pearson correlation analysis indicated that IL-6 and hs-CRP were positively correlated with miR-21 levels(P<0.05),while vitamin D and IL-10 were negatively correlated with miR-21 levels(P<0.05).Serum levels of miR-21,IL-6 and hs-CRP in the good prognosis group were lower than those in the poor prognosis group(P<0.05),while levels of vitamin D and IL-10 were higher(P<0.05).ROC curve analysis showed that the areas under the curve for serum miR-21,IL-6,hs-CRP,vitamin D,IL-10 and their combined prediction were all greater than 0.5,and the differences were statistically significant compared with the area under the reference line(P<0.05).Conclusion Serum exosomal miR-21 is highly expressed in female patients with T2DM complicated with CHD.IL-6 and hs-CRP are positively associated with serum exosomal miR-21 levels,and vitamin D and IL-10 are negatively correlated with serum exosomal miR-21 levels.关键词
糖尿病,2型/冠心病/女性/外泌体/白细胞介素6/维生素D/ROC曲线Key words
type 2 diabetes mellitus/coronary heart disease/female/exosomes/interleukin-6/vitamin D引用本文复制引用
苏曼,张学林,岳琳,关亚卿,刘淑艳,任丽萍,王翠英,范琳,姜萌..女性T2DM合并CHD患者血清外泌体miR-21表达情况及其与血清IL-6、维生素D水平的关系[J].转化医学杂志,2026,15(10):1658-1665,8.基金项目
河北省卫生健康委员会科研计划项目(20251154) （20251154）
石家庄市科技计划项目(231200273) （231200273）
