浙江农业学报2026，Vol.38Issue(8)：1517-1528,12.DOI:10.3969/j.issn.1004-1524.20250285
35 份贵州茶树种质资源的生化成分多样性分析与综合评价
Diversity analysis and comprehensive evaluation of biochemical components of 35 accessions of tea plants in Guizhou Province,China
摘要
Abstract
To explore the quality characteristics of biochemical components in natural hybrid progenies derived from several local landraces and tea cultivar Huangjinya,and to supply theoretical references for future novel tea cultivar breeding,high-performance liquid chromatography(HPLC)was adopted to quantify 28 biochemical components across 35 tea accessions conserved in the germplasm nursery of Guizhou Tea Research Institute.Multiple statistical approaches including genetic diversity analysis,correlation analysis,principal component analysis(PCA)and clus-ter analysis were integrated to conduct a comprehensive evaluation of all tested accessions.The results revealed that the average coefficient of variation(CV)of the 28 biochemical components reached 43.04％.Cysteine(Cys)con-tent exhibited the maximum CV of 117.40％,while caffeine(CAF)content showed the minimum CV of 14.55％.The mean value of the genetic diversity index was 1.81;phenylalanine(Phe)content possessed the highest index value of 2.09,and gallocatechin gallate(GCG)content had the lowest value of 0.66.Overall,amino acid compo-nents presented relatively high genetic diversity indexes.Seven principal components were extracted via PCA,ac-counting for a cumulative variance contribution rate of 78.451％.Total catechins(TC),arginine(Arg),epigallo-catechin gallate(EGCG),glutamic acid(Glu),epicatechin gallate(ECG)and theanine(The)contents were identified as the core evaluation indicators.All tested accessions were divided into 4 groups by cluster analysis.GroupⅣ,characterized by high amino acid contents as well as low alkaloid and catechin levels,obtained the optimal comprehensive score and possessed excellent suitability for manufacturing premium green tea.Based on the integrated evaluation results,9 elite accessions represented by GT-AS-70 and GT-AS-83 were screened for developing green tea-specific cultivars.关键词
茶树资源/生化成分/遗传多样性/主成分分析/聚类分析Key words
tea plant resource/biochemical component/genetic diversity/principal component analysis/cluster analysis分类
农业科技引用本文复制引用
郭维,马贤森,聂红艳,宋治豪,周轶,陈光勋,陈正武..35 份贵州茶树种质资源的生化成分多样性分析与综合评价[J].浙江农业学报,2026,38(8):1517-1528,12.基金项目
毕节市农业科学院科学技术项目(毕农科合[2025]3 号) （毕农科合[2025]3 号）
