浙江农业学报2026，Vol.38Issue(8)：1574-1588,15.DOI:10.3969/j.issn.1004-1524.20250455
亚洲莲全基因组SSR的基本特征与遗传变异
Basic characteristics and genetic variation of genome-wide simple sequence repeats in Asian lotus(Nelumbo nucifera)
摘要
Abstract
To gain deeper insights into the genetic characteristics of simple sequence repeat(SSR)across the whole genome of Asian lotus(Nelumbo nucifera),33 representative accessions were used as materials.Whole-genome re-sequencing was used to analyze genome-wide SSR loci and their motif features,and to investigate the associations be-tween SSR genetic variation and phenotypic traits including germplasm origin,flower color,petal type,and ecotype.The results showed that the average number of SSR loci in the Asian lotus genome was 64 253.8,with a range of 25 961 to 119 777.Among the eight SSR types,namely imperfect(c*),compound(c),and mononucleotide(p1)to hexanucleotide(p6)repeats,p1 and p2 SSRs were the most abundant,accounting for 55.38%and 29.91%of the total,respectively.SSR counts exhibited a gradually increasing trend from wild types to modern cultivars and landraces;the number of SSR loci in double-petal lotus was 83 298.6,which was 1.6 times that of the single-petal type(51 874.6).No significant difference in SSR number was observed between temperate and tropical lotus.Per-fect p1 to p6 SSRs contained an average of 2.0,4.0,10.0,23.6,49.8,and 88.6 motif types,respectively,with the three most abundant motifs being A/T(52.173%),AG/CT(16.971%),and AT/AT(11.358%)in de-scending order.This study delineated the detailed differences in genome-wide SSR counts and motif composition among representative Asian lotus individuals,revealed a highly conserved proportional pattern across the eight SSR types,and suggested that hybridization and artificial selection have promoted both an increase in the number of ge-nomic SSR loci and an enrichment of their motif diversity.关键词
莲属/亚洲莲/简单重复序列(SSR)/基序/花色/生态型Key words
Nelumbo/Asian lotus(Nelumbo nucifera)/simple sequence repeat(SSR)/motif/flower color/eco-type分类
农业科技引用本文复制引用
黄国洪,马艺妮,何佳欣,屠莉,廖伟彪,刘凤栾..亚洲莲全基因组SSR的基本特征与遗传变异[J].浙江农业学报,2026,38(8):1574-1588,15.基金项目
国家自然科学基金(32071821) （32071821）
