浙江医学2026，Vol.48Issue(17)：1825-1831,后插1,8.DOI:10.12056/j.issn.1006-2785.2026.48.17.2026-186
微RNA-25-3p调控PTEN/PI3K/AKT通路影响三阴性乳腺癌细胞增殖、迁移及侵袭的研究
Effects of microRNA-25-3p on the proliferation,migration and invasion of TNBC cells by regulating the PTEN/PI3K/AKT signal-ing pathway
摘要
Abstract
Objective To investigate the effects of microRNA-25-3p(miR-25-3p)on triple-negative breast cancer(TNBC)cell proliferation,invasion,and migration via regulating the phosphatase and tensin homolog(PTEN)/phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling axis.Methods Thirty TNBC patients undergoing modified radical mastectomy or breast-conserving surgery at Huzhou Maternal and Child Health Hospital from January to December 2025 were enrolled.TNBC tissues and adjacent normal tissues of patients were harvested.Quantitative real-time polymerase chain reaction(qRT-PCR)was performed to assess miR-25-3p expression in TNBC tissues and adjacent non-tumor tissues,as well as in MDA-MB-231 cells(TNBC cells)and MCF-10A cells(human normal breast cells).MDA-MB-231 cells were cultured in vitro by being transfected with miR-25-3p mimics,miR-25-3p inhibitor,or negative control(NC).MDA-MB-231 cell proliferation,migration,and invasion were evaluated using CCK-8,wound-healing,and Transwell assays,respectively.Western blotting was employed to detect PTEN,PI3K,phosphorylated PI3K(p-PI3K),AKT,and phosphorylated AKT(p-AKT)protein levels.A dual-luciferase reporter assay was conducted to verify the targeting relationship between PTEN and miR-25-3p.Results miR-25-3p expression was markedly higher in TNBC tissues than in matched adjacent tissues(P＜0.05),and its expression in MDA-MB-231 cells was higher than that in MCF-10A cells(P＜0.05).Knockdown of miR-25-3p substantially suppressed MDA-MB-231 cell proliferation,migration,and invasion,paralleled by upregulated PTEN expression and downregulated p-PI3K and p-AKT expressions(all P＜0.05).In contrast,overexpression of miR-25-3p resulted in enhanced cell proliferation,migration,and invasion of MDA-MB-231,diminished PTEN expression,and increased p-PI3K and p-AKT expressions(all P＜0.05).The dual-luciferase assay demonstrated that miR-25-3p could target and negatively modulate PTEN expression.Conclusion miR-25-3p promotes TNBC cell proliferation,migration,and invasion by targeting and suppressing PTEN gene expression,and activating the PI3K/AKT signaling pathway.关键词
三阴性乳腺癌/微RNA/磷酸肌醇-3激酶/增殖/侵袭Key words
Triple-negative breast cancer/MicroRNA/Phosphatidylinositol 3-kinase/Proliferation/Invasion引用本文复制引用
丁秀芳,陈莉杰,姚敏,王晨,邓再兴,张昕,纪存丽,关惠元,彭浦超..微RNA-25-3p调控PTEN/PI3K/AKT通路影响三阴性乳腺癌细胞增殖、迁移及侵袭的研究[J].浙江医学,2026,48(17):1825-1831,后插1,8.基金项目
浙江省自然科学基金项目(LY23H160005) （LY23H160005）
浙江省医药卫生科技计划项目(2024KY423) （2024KY423）
湖州市科技计划项目(2024GYB38) （2024GYB38）
