中兽医医药杂志2026，Vol.45Issue(5)：9-16,封2,9.DOI:10.13823/j.cnki.jtcvm.2026.044
黄精多糖调节巨噬细胞作用研究
The regulatory effect of Polygonatum polysaccharides on macrophages
摘要
Abstract
To investigate the immunomodulatory effect and underlying molecular mechanism of Polygonatum polysaccharide(PRP),RAW 264.7 macrophages were used as the cell model in this study.Real-time cell analysis(RTCA)was adopted to detect the stimulatory effects of varying concentrations of PRP on macrophage viability;the CCK-8 assay was performed to evaluate its cytotoxicity;phalloidin staining combined with laser scanning confocal microscopy was utilized to observe dynamic alterations in cytoskeletal proteins;and transcriptome sequencing coupled with cluster analysis was applied to screen differentially expressed genes(DEGs)and explore relevant signaling pathways.The results demonstrated that treatment with 200 μg/mL PRP persistently maintained high viability of macrophages without inducing cytotoxicity.PRP markedly triggered plasma membrane ruffling and pseudopod extension,elevated the fluorescence intensity on cell membranes,and sustained active status of cytoskeletal proteins within 40 hours.Transcriptomic profiling revealed that DEGs were predominantly enriched in the Toll-like receptor signaling pathway,antigen processing and presentation,as well as cell adhesion molecule-associated pathways,functionally participating in peptide biosynthesis,amide biosynthesis,peptide metabolism and cellular amide metabolism.In conclusion,PRP effectively enhances the immunocompetence of macrophages primarily via activating the Toll-like receptor signaling pathway and modulating substance metabolism.This research provided experimental and molecular evidence for the development and application of PRP as an immunomodulator.关键词
黄精多糖/巨噬细胞/免疫调节/转录组测序/Toll样受体信号通路Key words
Polygonatum polysaccharide/macrophages/immunomodulation/transcriptome sequencing/Toll-like receptor signaling pathway分类
农业科技引用本文复制引用
张岑容,李永久,黄志,潘阳明,苏菲..黄精多糖调节巨噬细胞作用研究[J].中兽医医药杂志,2026,45(5):9-16,封2,9.基金项目
浙江省农业重大技术协同推广计划项目 （）
