中兽医医药杂志2026，Vol.45Issue(5)：17-25,封3,10.DOI:10.13823/j.cnki.jtcvm.2026.051
基于代谢组学研究绞股蓝提取物对巴马香猪肠道代谢的调控作用
Regulatory effect of Gynostemma pentaphyllum extract on intestinal metabolism in Bama Xiang pigs based on metabolomics
摘要
Abstract
This study aimed to investigate the regulatory effects of Gynostemma pentaphyllum extract(GPE)on the intestinal metabolome of Bama Xiang piglets.Fifty 30-35-day-old Bama Xiang piglets were randomly assigned to one control group and three GPE-supplemented groups(low,medium and high doses).Piglets in the control group received a basal diet,whereas the three treatment groups were fed basal diets supplemented with 0.4%,0.8%and 1.6%GPE,respectively for a 28-day experimental period.The findings indicated that dietary GPE supplementation significantly altered the intestinal metabolite profile of piglets(P≤0.05).Differential metabolite analysis revealed that high-dose GPE upregulated the levels of organic acid derivatives,lipids and organic nitrogen compounds,and downregulated the contents of N-acetyl-D-galactosamine and DL-proline.KEGG pathway enrichment analysis demonstrated that compared to the control group,GPE significantly modulated pathways involved in lipid metabolism,amino acid metabolism,nucleotide metabolism,as well as cofactor and vitamin metabolism(P≤0.05),with more pronounced effects observed in the medium-and high-dose groups.Pearson correlation analysis showed that metabolites including tomatidine and acetylcholine were positively correlated with beneficial taxa such as Bacteroidetes and Lactobacillus,whereas stearic acid was negatively correlated with Verrucomicrobia and Ruminococcaceae UCG-002.These beneficial microbes upregulated antioxidant biomarkers(SOD,GSH-Px)and the expression of tight junction proteins,suppressed the expression of pro-inflammatory genes(IL-1β,TNF-α)and reduced MDA accumulation(P≤0.05).In conclusion,dietary GPE improves intestinal health of Bama Xiang piglets via modulating gut microbiota-metabolite crosstalk,optimizing core metabolic pathways,elevating antioxidant capacity,mitigating intestinal inflammation and preserving intestinal barrier integrity,medium and high inclusion levels exert preferable efficacy.关键词
绞股蓝提取物/巴马香猪仔猪/肠道健康/代谢组学Key words
Gynostemma pentaphyllum extract/Bama Xiang piglets/gut health/metabolomics分类
农业科技引用本文复制引用
余善,陈思羽,施君,周淑棉,胡庭俊..基于代谢组学研究绞股蓝提取物对巴马香猪肠道代谢的调控作用[J].中兽医医药杂志,2026,45(5):17-25,封3,10.基金项目
国家自然科学基金项目(32560871) （32560871）
广西大学巴马产教融合研究院人才科技专项(20220024) （20220024）
