作物学报2026，Vol.52Issue(9)：2535-2548,14.DOI:10.3724/SP.J.1006.2026.53100
植物原生质体制备优化及其单细胞电感耦合等离子体质谱应用挑战
Optimization of plant protoplast preparation and application challenges of SC-ICP-MS
摘要
Abstract
Single cell-inductively coupled plasma-mass spectrometry(SC-ICP-MS)can accurately analyze the element distribu-tion in plant tissues at the single-cell level.This technology can explore the cell heterogeneity ignored by traditional population analysis methods,significantly improve the spatial resolution of elemental analysis,and provide a new way for elemental quanti-fication.However,SC-ICP-MS determination technology is limited by the acquisition of high-quality cell suspension and accurate elemental analysis.Current studies have shown that the preparation of high-quality protoplasts is a critical prerequisite for the application of this technology.Therefore,the establishment of an efficient and stable plant protoplast preparation method directly determines the sensitivity,quantitative accuracy and repeatability of SC-ICP-MS detection,which has become a prerequisite for restricting the application of this technology.In this review,the preparation process of common plant protoplasts and their core adaptation principles to SC-ICP-MS were introduced,including source tissue selection,pretreatment,optimization of enzymatic hydrolysis system(ratio of enzymatic hydrolysate,digestion time,osmotic pressure regulation and addition of auxiliary sub-stances),protoplast purification,vitality detection,and chemical fixation.The cell-level research on nutrients and heavy metals was summarized as well as the parameter optimization strategies of SC-ICP-MS were summarized.This technique is still faced with challenges in a proper balance between cell integrity and element retention,besides the possibility of accounting for the to-xicity effects,uptake,and biological effects of exogenous substances.Future research should combine imaging,omics and other technologies to explore the metabolic process of elements,and further promote the application of SC-ICP-MS detection in agri-culture,environment,ecology and life sciences.关键词
单细胞电感耦合等离子体质谱/原生质体制备/植物细胞元素/细胞异质性/技术参数优化Key words
SC-ICP-MS/protoplast preparation/plant cell elements/cell heterogeneity/optimization of technical parameter引用本文复制引用
贾梦辰,王恩慧,于小晶,李顺,白利勇,戴九兰..植物原生质体制备优化及其单细胞电感耦合等离子体质谱应用挑战[J].作物学报,2026,52(9):2535-2548,14.基金项目
本研究由山东省自然科学基金项目(ZR2025MS533),青岛市博士后项目(QDBSH20250102005),山东大学微生物技术国家重点实验室生命科学领域校内联合项目基金(SKLMTIJP-2024-06),国家自然科学青年科学基金项目(42507017)和龙口市 2024 年花生品种实验技术项目(14600240032)资助.This study was supported by the Natural Science Foundation of Shandong Province(ZR2025MS533),the Qingdao Postdoctoral Project(QDBSH20250102005),the Shandong University State Key Laboratory of Microbial Technology Joint Project Fund in the Field of Life Sci-ences(SKLMTIJP-2024-06),the National Natural Science Youth Science Foundation of China(42507017),and the Longkou City 2024 Pea-nut Variety Experimental Technology Project(14600240032). （ZR2025MS533）
