作物学报2026，Vol.52Issue(9)：2573-2583,11.DOI:10.3724/SP.J.1006.2026.64016
甘薯苗期耐低钾性状GWAS分析及候选基因筛选
GWAS analysis of low potassium tolerance trait at seedling stage in sweetpo-tato and screening of candidate genes
摘要
Abstract
Sweetpotato is a typical"potassium(K+)-loving"crop.Exploring genetic resources of low-K+-tolerance and elucidating the underlying mechanisms in sweetpotato are important strategies to address soil potassium deficiency and the shortage of potas-sium resources.This study aimed to screen candidate genes associated with low-K+tolerance in sweetpotato through ge-nome-wide association analysis(GWAS),providing a reference for breeding low-K+-tolerant varieties and mining genes for effi-cient K+utilization.A total of 213 sweetpotato varieties(lines)were subjected to hydroponic experiments under low-K+stress(0.1 mmol L-1 K+)and normal K+treatment(10.0 mmol L-1 K+).Traits including biomass,K+content,K+accumulation,K+use efficiency,and relative growth rate were collected for GWAS.Results showed that six traits—dry biomass,fresh biomass,relative growth rate,shoot K+accumulation,root K+accumulation,and whole plant K+accumulation—were significantly or extremely significantly correlated with each other.Through GWAS,seven genomic segments associated with five traits(relative growth rate,shoot K+content,root K+accumulation,plant K+accumulation,and dry biomass)were identified,containing a total of 35 SNP loci.Based on core SNP loci and gene function annotation,7 candidate genes related to low-K+-tolerance in sweetpotato were predicted.RT-qPCR verification discovered that three candidate genes,itf12g23740.t1,itf08g06140.t1,and itf08g06320.t1-encoded cation/H+exchanger 15(CHX15),auxin receptor F-box protein 5(AFB5),and vernalization-insensitivity 3-like 1(VIL1),respectively,showed expression differences among varieties with contrasting low-K+-tolerance.Overall,a total of 35 significant SNP loci associated with low-K+tolerance traits were detected in the 213 sweetpotato accessions,and seven candidate genes were predicted.Among them,itf12g23740.t1,itf05g03220.t1,and itf08g06140.t1 are important candidate genes for low-K+tolerance in sweetpotato and warrant further investigation.关键词
甘薯/GWAS/低钾胁迫/基因功能注释/基因挖掘Key words
sweetpotato/GWAS/low-K+stress/gene function annotation/gene mining引用本文复制引用
刘明,唐忠厚,林琳,于永超,靳容,赵鹏,张强强,朱晓亚,王静,李硕..甘薯苗期耐低钾性状GWAS分析及候选基因筛选[J].作物学报,2026,52(9):2573-2583,11.基金项目
本研究由国家重点研发计划项目(2025YFD2300600,2024YFD2301000),农业农村部甘薯生物学与遗传育种重点实验室开放课题(KF2024001)和财政部和农业农村部国家现代农业产业技术体系(甘薯,CARS-10)资助.This study was supported by the National Key Research&Development Program of China(2025YFD2300600,2024YFD2301000),the Open Project of the Key Laboratory of Sweetpotato Biology and Genetic Breeding of the Ministry of Agriculture and Rural Affairs(KF2024001),and the China Agriculture Research System of MOF and MARA(Sweetpotato,CARS-10). （2025YFD2300600,2024YFD2301000）
