花生蔗糖含量相关基因的定位及KASP标记的开发与验证
Mapping of genes-related to sucrose content in peanut and development and validation of KASP markers in peanut
摘要
Abstract
In this study,an F2 segregation population derived from a cross between the high-sucrose peanut variety"Jihuatian 1"and the low-sucrose peanut line"JM135"was utilized to map QTLs associated with sucrose content via BSA resequencing.The results showed that the kernel sucrose content in the F2 population ranged from 2.89%to 8.54%,exhibiting continuous variation and a normal distribution.Through BSA-seq analysis,six candidate intervals were initially identified,on chromosomes Arahy.04 and Arahy.16.Two KASP markers,16-1475 and 16-1522,were developed for marker-assisted selection(MAS)of the kernel su-crose content in peanut.Based on gene annotation information,two candidate genes were screened.Expression profile indicated that Arahy.RUR3FB and Arahy.LJ1TN7 are potential candidate genes regulating sucrose content in peanuts.This study provides theoretical support for mining genes related to sucrose content in peanuts and offers technical support for breeding new high sugar peanut varieties through molecular breeding approaches.关键词
花生/蔗糖含量/BSA-seq/基因定位/KASP标记Key words
peanut/sucrose content/BSA-seq/gene mapping/KASP marker引用本文复制引用
潘丽娟,迟晓元,姜骁,许静,殷祥贞,万菲菲,孙淑娟,赵旭红,陈娜,马俊卿..花生蔗糖含量相关基因的定位及KASP标记的开发与验证[J].作物学报,2026,52(9):2598-2607,10.基金项目
本研究由山东省重点研发计划项目(2025CXPT167,2025LZGC020),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13),山东省农业科学院农业科技创新工程项目(CXGC2026C41,CXGC2025F19,CXGC2025C19),泰山学者工程项目(tstp20240523,tsqn202312292),山东省自然科学基金项目(ZR2023QC177,ZR2023QC146,ZR2025QC282),农业农村部油料作物生物学与遗传育种重点实验室项目(KF2024007)和青岛市自然科学基金青年项目(25-1-1-38-zyyd-jch)资助.This study was supported by the Key Research and Development Project of Shandong Province(2025CXPT167,2025LZGC020),the China Agriculture Research System of MOF and MARA(CARS-13),the Innovation Project of SAAS(CXGC2026C41,CXGC2025F19,CXGC2025C19),the Taishan Scholars Program(tstp20240523,tsqn202312292),the Natural Science Fund of Shandong Province(ZR2023QC177,ZR2023QC146,ZR2025QC282),the Open Project of Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs(KF2024007),and the Qingdao Natural Science Foundation Youth Project(25-1-1-38-zyyd-jch). （2025CXPT167,2025LZGC020）
