植物保护学报2026，Vol.53Issue(4)：881-891,11.DOI:10.13802/j.cnki.zwbhxb.2026.2025173
RNAi在害虫防治中的脱靶效应及其规避策略
Off-target effects and mitigation strategies of RNA interference in pest control
摘要
Abstract
RNA interference(RNAi),as a precise gene-silencing technology,has shown great potential for application in sustainable pest management.However,the biosafety risks posed non-target organ-isms by RNAi off-target effects have become a major constraint on its widespread application.This review systematically summarizes the molecular mechanisms underlying RNAi off-target effects,classi-fying them into sequence-specific and sequence-independent categories.These mechanisms involve sequence similarity,base-pairing characteristics,gene transcription levels,small interfering RNA(siRNA)/double-stranded RNA(dsRNA),microRNA(miRNA),and the loading fidelity of the RNA-induced silencing complex(RISC).The key factors influencing off-target effects,including dsRNA structural features,species differences,and dsRNA delivery methods,are also summarized.In addition,current mitigation strategies,such as optimizing dsRNA design,chemically modifying siRNA,and applying off-target prediction software and webbased evaluation tools,are discussed.This review pro-vides a theoretical basis for the safe application of RNAi technology in pest control.关键词
RNA干扰/脱靶效应/非靶标生物/应对策略/害虫防治Key words
RNA interference/off-target effect/non-target organism/mitigation strategy/pest control引用本文复制引用
陈嘉生,黄云,李秀霞,彭英传,王康旭,徐鹿,盛成旺..RNAi在害虫防治中的脱靶效应及其规避策略[J].植物保护学报,2026,53(4):881-891,11.基金项目
安徽省农学相关领域研究生创新(创业)竞赛项目(2024cxcyjs011),安徽省自然科学基金青年项目(2408085QC096),国家自然科学基金面上项目(31972309) （创业）
