植物保护学报2026，Vol.53Issue(4)：938-946,9.DOI:10.13802/j.cnki.zwbhxb.2026.2026010
稻瘟病菌内切几丁质酶基因MoENCH1的功能分析
Functional analysis of the endochitinase gene MoENCH1 in rice blast fungus Magnaporthe oryzae
摘要
Abstract
To investigate the function of the endochitinase(ENCH)gene MoENCH1 in rice blast fungus Magnaporthe oryzae,a MoENCH1 knockout mutant was generated using the split-marker approach,and a complemented strain was subsequently constructed.The biological functions of MoENCH1 were systematically characterized,and the molecular mechanisms underlying its role in pathogenicity were further investigated with transcriptome sequencing.The results showed that,compared with the wild-type strain,the MoENCH1 knockout mutant significantly reduced growth rates on oatmeal agar(OMA),complete medium,and minimal medium.Sporulation on OMA was significantly decreased,accompa-nied by reduced conidial germination and appressorium formation rates.In addition,the mutant was insensitive to the cell wall stress agent Congo red and exhibited significantly reduced pathogenicity toward both barley and rice.Transcriptome analysis indicated that MoENCH1 is involved in regulating pathways related to autophagy,peroxisomes,ubiquitin-mediated proteolysis,and glycerophospholipid metabolism in M.oryzae.These results demonstrate that MoENCH1 plays important roles in regulating hyphal growth,sporulation,conidial germination,appressorium formation,and pathogenicity in M.oryzae.关键词
稻瘟病菌/几丁质内切酶/致病力/转录组/产孢/孢子萌发/附着胞形成Key words
Magnaporthe oryzae/endochitinase/pathogenicty/RNA-Seq/sporulation/conidial germi-nation/appressorium formation引用本文复制引用
赵娟,许立鹤,石鑫怡,邹欣梅,吕柔柔,赵鑫,刘杰..稻瘟病菌内切几丁质酶基因MoENCH1的功能分析[J].植物保护学报,2026,53(4):938-946,9.基金项目
国家自然科学基金项目(32402340),四川省自然科学基金项目(2024NSFSC1320) （32402340）
