植物保护学报2026，Vol.53Issue(4)：947-954,8.DOI:10.13802/j.cnki.zwbhxb.2026.2026011
甘肃省定西市玉米坏死条纹病毒分离物的分子鉴定及全基因组序列分析
Molecular identification and complete genome sequence analysis of a maize necrotic streak virus isolate from Dingxi,Gansu Province,China
摘要
Abstract
To identify the pathogen associated with chlorotic streak symptoms in maize(Zea mays)in Dingxi City,Gansu Province,high-throughput sequencing combined with reverse transcription-poly-merase chain reaction(RT-PCR)and 5′/3′ rapid amplification of cDNA ends(5′/3′ RACE)was used to identify the virus and determine its complete genome sequence.Sequence characteristics and phyloge-netic relationships were analyzed using NCBI BLAST,MegAlign,and IQ-TREE.RT-PCR was further used to investigate the occurrence and distribution of the virus in different regions of Gansu Province.The results showed that maize necrotic streak virus(MNeSV)was identified from symptomatic maize samples collected in Dingxi City and the isolate was designated MNeSV-DX.The complete genome of MNeSV-DX was 4 094 nt in length and contained five predicted open reading frames.Its genomic orga-nizationwas identical to that of the US isolates(GenBank accession numbers NC_007729.1 and AF266518.2).The complete genome shared 98.8％nucleotide sequence identity and 99.0％amino acid sequence identity with these reference isolates.Phylogenetic analysis demonstrated that the three iso-lates clustered within the same clade,indicating a close evolutionary relationship with limited genetic divergence.RT-PCR detection further demonstrated the presence of MNeSV in maize samples collected from Dingxi,Gannan,and Longnan in Gansu Province.These findings indicate that MNeSV can natu-rally infect maize in China and is closely associated with the chlorotic streak symptoms observed in Dingxi City,highlighting the need for increased attention to the occurrence and spread of this virus.关键词
玉米/玉米坏死条纹病毒/病毒鉴定/进化分析/发生/分布Key words
maize/maize necrotic streak virus(MNeSV)/virus identification/phylogenetic analysis/occurrence/distribution引用本文复制引用
房静静,王嘉荟,胡小平,马悦,徐亨昊,张树武,徐秉良,薛应钰,梁巧兰,牛二波..甘肃省定西市玉米坏死条纹病毒分离物的分子鉴定及全基因组序列分析[J].植物保护学报,2026,53(4):947-954,8.基金项目
国家重点研发计划项目(2025YFC2609000),甘肃省青年科技基金项目(23JRRA1431),甘肃农业大学人才启动金项目(GAU-KYQD-2022-28),宁夏农业科技自主创新资金专项(NKYG-25-11),甘肃农业大学植物病害绿色防控团队建设项目(26000102) （2025YFC2609000）
