植物遗传资源学报2026，Vol.27Issue(9)：1710-1722,13.DOI:10.13430/j.cnki.jpgr.20260427006
大豆种质资源老化过程中黄酮类物质变化特征
Variation Characteristics of Flavonoids in Soybean Germplasm Resources During Aging
摘要
Abstract
To clarify the variation characteristics of flavonoids during the aging process of soybean germplasm resources and their relationship with germplasm vigor,this study used 27 soybean resources from different origins as experimental materials.Artificial aging treatment at 40℃ was applied to obtain different vigor gradients,and the relationships between germplasm vigor indicators,total flavonoid content,and changes in key flavonoid compounds were analyzed.The results showed that:the vigor loss characteristics varied among different accessions;the initial total flavonoid content differed significantly among accessions(0.373-3.381 mg/g),and most accessions showed an intial upward trend followed by a decrease during aging,which was extremely significantly negatively correlated with germination rate.HPLC-MS/MS(High performance liquid chromatography-tandem mass spectrometry)was used to analyze the flavonoid composition of three representative germplasm accessions with high,medium and low seed vigor levels,and combined with principal component analysis,cluster analysis,partial least squares discriminant analysis,and correlation analysis,key differential flavonoid compounds including 7,4'-dihydroxyflavone,calycosin,genistein,apigenin and alpinetin were identified,which were highly significantly negatively correlated with germination rate.These key flavonoid compounds were significantly enriched in low-vigor samples and could be used to distinguish soybean germplasm with different vigor levels.Therefore,flavonoid compounds can serve as potential biochemical indicators for evaluating soybean germplasm vigor,providing a theoretical basis for the safe conservation and utilization of germplasm resources.关键词
大豆/种质资源/活力/老化/黄酮类物质Key words
soybean/germplasm resources/vigor/aging/flavonoids引用本文复制引用
董娜,杨海峰,尹广鹍,李洋,张金梅,辛霞..大豆种质资源老化过程中黄酮类物质变化特征[J].植物遗传资源学报,2026,27(9):1710-1722,13.基金项目
国家重点研发计划(2024YFD1200100) （2024YFD1200100）
中国农业科学院科技创新工程(CAAS-ASTIP-2026-ICS01)National Key Research and Development Program of China(2024YFD1200100) （CAAS-ASTIP-2026-ICS01）
Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2026-ICS01) （CAAS-ASTIP-2026-ICS01）
