植物遗传资源学报2026，Vol.27Issue(9)：1772-1780,9.DOI:10.13430/j.cnki.jpgr.20260407001
水稻种质资源硝磺草酮抗性鉴定与评价
Identification and Evaluation of Mesotrione Resistance in Rice Germplasm Resources
摘要
Abstract
Weed is a major factor affecting rice yield and quality,and breeding herbicide-resistant rice varieties is an effective approach for weed control.Mesotrione is a 4-hydroxyphenylpyruvate dioxygenase-inhibiting herbicide with great application potential because of its broad-spectrum weed-control,safety to succeeding crops,and the low likelihood of resistance development in weeds.Natural alleles conferring mesotrione resistance are present in rice germplasm resources.This study first employed a liquid culture-based evaluation method to determine 0.2 μmol/L mesotrione as the screening concentration for extremely sensitive materials and 0.8 μmol/L mesotrione as the screening concentration for resistant materials.Then,based on the resistance response of different rice germplasm resources to mesotrione,resistance was classified into levels 1,3,5,7,and 9.Among 760 rice germplasm resources,19 extremely sensitive germplasm with resistance level 9 were selected using 0.2 μmol/L mesotrione concentration.And then the mesotrione concentration was increased to 0.8 μmol/L,four highly resistant germplasm with resistance level 1 and 28 moderately resistant germplasm with resistance level 3 from the 760 germplasm resources were selected.Further field spraying experiments at the seedling and tillering stages showed that the highly resistant germplasm screened in this study could tolerate up to 80 mL/667 m2 of 15％mesotrione suspension concentrate spray in field test.The liquid culture mesotrione resistance identification method established in this study enable rapid and large-scale evaluation of mesotrione resistance in rice germplasm resources.The highly resistant and highly susceptible germplasm identified in this study not only provide excellent genetic resources for breeding new mesotrione-resistant rice varieties,but also offer important materials for resistance gene mining and genetic mechanism analysis.关键词
水稻/种质资源/除草剂/硝磺草酮/抗性鉴定Key words
rice/germplasm resources/herbicide/mesortrione/resistance identification引用本文复制引用
王培娜,葛丽云,孙家骏,陈卓,李苇菡,魏义群,杜彦修,李俊周,孙红正..水稻种质资源硝磺草酮抗性鉴定与评价[J].植物遗传资源学报,2026,27(9):1772-1780,9.基金项目
农业农村部种质资源鉴定项目(19230590) （19230590）
河南省自然科学基金(252300423051) （252300423051）
河南现代农业水稻产业技术体系(HARS-22-03-G3)Germplasm Resource Identification Project of the Ministry of Agriculture and Rural Affairs(19230590) （HARS-22-03-G3）
Natural Science Foundation of Henan Province(252300423051) （252300423051）
Henan Modern Agriculture Rice Industry Technology System(HARS-22-03-G3) （HARS-22-03-G3）
