中西医结合肝病杂志2026，Vol.36Issue(8)：967-972,6.DOI:10.3969/j.issn.1005-0264.2026.008.005
祛湿化瘀方治疗MCD饮食诱导的代谢功能障碍相关脂肪性肝炎小鼠的药效观察
Pharmacological effects of Qushi Huayu formula in improving MCD diet-induced metabolic dysfunction-associated steatohepatitis in mice
摘要
Abstract
Objective:To investigate the effects of Qushi Huayu formula(QSHY)on a methionine-choline deficient(MCD)diet-induced mouse model of metabolic dysfunction-associated steatohepatitis(MASH).Methods:Twenty-four mice were randomly divided into two groups:the methionine-choline sufficient(MCS)diet group(n=6)and the MCD diet model group(n=18).At the end of 3 weeks of modeling,the MCD diet-fed mice were further randomized into three groups:MCD group,MCD+QSHY group,and MCD+obeticholic acid(OCA)group.The mice received corresponding drug treatments or vehicle via oral gavage for 4 weeks before sample collection.General conditions of the mice were observed;liver triglyceride(TG)content and serum Alanine Aminotransferase(ALT)、Aspartate Aminotransferase(AST)levels were measured.Hepatic histopathological changes were evaluated using H&E staining,Oil Red O staining,and Sirius red staining.The mRNA expression levels of genes related to lipid synthesis(Fatty Acid Synthase[FASN],Sterol Regulatory Element Binding Transcription Factor 1[SREBF-1]),inflammation(Tumor Necrosis Factor-α[TNF-α],Interleukin[IL]-1β,IL-6),and fibrosis(Collagen Type Ⅰ Alpha 1[COL1A1],Actin Alpha 2 Smooth Muscle[ACTA2])were detected by quantitative real-time polymerase chain reaction(qRT-PCR).Results:Compared with the MCS group,the MCD group exhibited significantly increased hepatic TG content(P＜0.01),decreased serum TG content(P＜0.001),and significantly elevated serum ALT and AST levels(P＜0.001).Oil Red O staining revealed extensive lipid droplet accumulation in the liver,while H&E staining showed prominent hepatic steatosis and inflammatory cell infiltration.Sirius Red staining demonstrated obvious collagen deposition.qRT-PCR analysis showed that the mRNA expression levels of the lipogenesis-related genes FASN and SREBF-1,the inflammatory cytokines TNF-α,IL-1β and IL-6,as well as the fibrosis-related genes COL1A1 and ACTA2 were significantly increased(P＜0.01).Compared with the MCD group,both the MCD+QSHY and MCD+OCA groups exhibited significantly reduced hepatic TG content(P＜0.05),while serum TG levels were significantly increased in the MCD+QSHY group(P＜0.001).Serum ALT and AST levels were markedly decreased in both treatment groups(P＜0.05).Oil Red O staining showed markedly reduced hepatic lipid accumulation following QSHY or OCA treatment.H&E staining showed that both interventions alleviated hepatic steatosis and inflammatory infiltration,while Sirius Red staining revealed markedly reduced collagen deposition.Furthermore,both treatments downregulated the expression of FASN,SREBF-1,TNF-α,IL-6,COL1A1,and ACTA2(P＜0.05).Conclusion:Qushi Huayu Formula exhibits significant ameliorative effects on MASH mice induced by MCD diet,reducing hepatic lipid accumulation and pathological damage in liver tissue while suppressing liver inflammation and fibrosis.关键词
祛湿化瘀方/蛋氨酸胆碱缺乏/代谢功能障碍相关脂肪性肝炎Key words
Qushi Huayu formula/methionine-choline deficient/metabolic dysfunction-associated steatohepatitis分类
医药卫生引用本文复制引用
于楚楚,潘雨晴,毛傲洁,赵瑜,胡义扬,刘巧红..祛湿化瘀方治疗MCD饮食诱导的代谢功能障碍相关脂肪性肝炎小鼠的药效观察[J].中西医结合肝病杂志,2026,36(8):967-972,6.基金项目
国家自然科学基金资助项目(No.82204840,82174186),上海市科学技术委员会扬帆计划(No.22YF1449400) （No.82204840,82174186）
