中西医结合肝病杂志2026，Vol.36Issue(8)：978-981,4.DOI:10.3969/j.issn.1005-0264.2026.008.007
丙型肝炎合并艾滋病肝郁脾虚证患者血液代谢组学研究
Characteristics of blood metabolomics in HCV/AIDS-coinfected patients with liver hyperactivity with spleen deficiency pattern
摘要
Abstract
Objective:This study explored the characteristics of blood metabolomics of hepatitis C virus(HCV)/human immunodeficiency virus(HIV)-coinfected patients with liver hyperactivity with spleen deficiency pattern.Methods:Fasting blood samples were collected from hepatitis C complicated with acquired immunodeficiency syndrome patients with liver hyperactivity with spleen deficiency pattern and healthy individuals,with the blood of healthy people as the control group.High-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)metabolomics technology was used to detect the metabolites of the two groups.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)were performed to screen the differential metabolites between different groups.Results:In this investigation,patients with HIV/HCV liver-qi depression and spleen-deficiency syndrome could be easily distinguished from healthy people according to the metabolic profiles.There was obvious lipid aggregation in the bodies of patients with HIV/HCV liver-qi depression and spleen-deficiency syndrome.The main types of metabolic differences were glycerophospholipids,fatty acids and their derivatives.Through OPLS-DA(Orthogonal Partial Least Squares Discriminant Analysis)screening,the differential metabolites with VIP value＞1.0,AUC(Area Under ROC Curve)value＞0.9 and P value＜0.05 were screened out.There were 23 differential metabolites in total.The up-regulated metabolites in the two groups were catechin 3-tetradecanoate,sorbitol palmitate,decanoate,hexylbenzene and fluocinolone acetonide.The down-regulated metabolites were glycerides,chromanol,isopentenol,acylcarnitine,benzamide,palonosetron,fatty acids,fatty acid conjugates,piperazinecarboxamide,steroids,aryl ketones,trans-2-hexadecenoyl-carnitine,phenylpropane,steroid lactones,dextrorphan,glycerophosphoethanolamine,glycerophospholipids and lysoenzymes.The involved metabolic pathways were mainly the glycerol and fatty acid metabolic pathways.Conclusion:Metabolomics technology can reveal the metabolic differences between patients with HCV/AIDS liver-qi depression and spleen-deficiency syndrome and healthy people,which can provide a relevant reference for the differentiation of traditional Chinese medicine syndrome types of HIV/HCV.关键词
代谢组学/艾滋病/丙型肝炎/肝郁脾虚证/中医证候Key words
metabolomics/acquired immunodeficiency syndrome/hepatitis C virus/liver hyperactivity with spleen deficiency pattern/traditional Chinese medicine syndromes分类
医药卫生引用本文复制引用
高文俊,蒲亚萍,夏海梅,陆中云,王莉..丙型肝炎合并艾滋病肝郁脾虚证患者血液代谢组学研究[J].中西医结合肝病杂志,2026,36(8):978-981,4.基金项目
云南省中医联合专项青年项目(No.202101AZ070001-281),云南省中医联合会专项重点项目(No.202301AZ070001-12),云南省科技厅-云南中医药大学应用基础研究联合专项资金面上项目(No.202101AZ070001-062),云南省中医临床优秀人才研修项目(云卫中医综合发[2024]1号) （No.202101AZ070001-281）
