中药材2026，Vol.49Issue(3)：577-581,5.DOI:10.13863/j.issn1001-4454.2026.03.004
锐尖山香圆离体快繁技术研究
Study on In Vitro Rapid Propagation Technology of Turpinia arguta
摘要
Abstract
Objective:To establish an in vitro rapid propagation technology system for Turpinia arguta,so as to provide technical support for the large-scale propagation of tissue culture seedlings.Methods:Using the tender branches of the axillary buds of Turpinia arguta as explants,the best axillary bud initiation medium,advanced-bud induction and proliferation medium and rooting medium were screened out.The axillary bud induction rate,advanced-bud induction rate,proliferation coefficient,rooting rate and transplantation sur-vival rate were statistically analyzed.Results:The optimal axillary bud initiation medium for Turpinia arguta was MS+1.0 mg/L 6-BA+0.05 mg/L NAA,and the axillary bud induction rate reached 88.33%.The optimal medium for adventitious bud induction and prolifer-ation was MS+0.2 mg/L 6-BA+0.05 mg/L GA3+0.03 mg/L NAA,with a proliferation coefficient of 3.87.The optimal rooting medium was 1/2MS+2.0 mg/L IAA+0.5 mg/L NAA+0.2 g/L AC,with a rooting rate of 92.67%and a survival rate of 98.56%after trans-plantation.Conclusion:The in vitro rapid propagation technology system of Turpinia arguta has been successfully established.关键词
锐尖山香圆/离体快繁/不定芽/增殖/生根Key words
Turpinia arguta(Lindl.)Seem./In vitro rapid propagation/Adventitious bud/Proliferation/Rooting分类
医药卫生引用本文复制引用
戴小英,张云童,徐小强,程强强,陶秀花,刘新亮..锐尖山香圆离体快繁技术研究[J].中药材,2026,49(3):577-581,5.基金项目
江西省重大科技研发专项项目(20233AAE02017) （20233AAE02017）
江西省林业局科技创新重点攻关项目(创新专项[2023]1号) （创新专项[2023]1号）
江西省林科院基础研究与人才科研专项(2023522001) （2023522001）
