中医临床研究2026，Vol.18Issue(17)：20-29,10.DOI:10.3969/j.issn.1674-7860.2026.17.003
基于网络药理学和分子对接探讨增视明目方治疗近视的作用机制
Exploring the mechanism of Zengshi Mingmu Fang in treating myopia based on network pharmacology and molecular docking
易沛言 1龙柯君 1彭佳蕊 1陈书瑶 1韩琼钰 1陈向东1
作者信息
- 1. 湖南中医药大学,湖南 长沙,410208||湖南中医药大学第一附属医院,湖南 长沙,410007
- 折叠
摘要
Abstract
Objective:To analyze and explore the mechanism by which Zengshi Mingmu Fang(增视明目方)regulates the progression of myopia by means of network pharmacology and molecular docking.Methods:Active compounds and their putative targets of Zengshi Mingmu Fang were retrieved from TCMSP.Myopia-related genes were collected from the GeneCards,OMIM.Overlapping targets between the Zengshi Mingmu Fang and the disease were identified with Venny 2.1.0.A protein-protein interaction network was constructed via STRING to screen potential hub targets.Molecular docking was performed with AutoDock Vina and visualized in PyMOL.Gene ontology(GO)functional annotation and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment were conducted for the hub targets.Finally,the herb-compound-target-disease network was plotted with Cytoscape 3.10.2 and its plug-ins.Results:A total of 130 bioactive ingredients and 976 putative targets related to the Zengshi Mingmu Fang,together with 5 901 genes associated with myopia,were retrieved.Subsequent analysis identified 468 potential therapeutic targets of Zengshi Mingmu Fang against myopia.The core active components were kaempferol,quercetin,isorhamnetin and gardenin.A total of 89 hub targets(including AKT1,TP53,TNF,IL-6 and ALB)were screened,mainly involved in apoptosis regulation and the PI3K-Akt signaling pathway.Molecular-docking assays showed binding energies below-20.92 kJ/mol for all core pairs,indicating stable binding affinity.Conclusion:Zengshi Mingmu Fang may synergistically intervene in myopia through multiple components,multiple targets and multiple pathways,providing a theoretical basis for further drug development.关键词
网络药理学/分子对接/增视明目方/近视Key words
Network pharmacology/Molecular docking/Zengshi Mingmu Fang/Myopia分类
医药卫生引用本文复制引用
易沛言,龙柯君,彭佳蕊,陈书瑶,韩琼钰,陈向东..基于网络药理学和分子对接探讨增视明目方治疗近视的作用机制[J].中医临床研究,2026,18(17):20-29,10.
