中医临床研究2026，Vol.18Issue(19)：48-58,11.DOI:10.3969/j.issn.1674-7860.2026.19.006
基于网络药理学与分子对接技术探索清润四边汤外用治疗外阴硬化性苔藓的作用机制研究
Exploring the mechanism of action of Qingrun Sibian decoction in treating vulvar lichen sclerosus by external application:a study based on network pharmacology and molecular docking
摘要
Abstract
Objective:To elucidate the therapeutic mechanisms of Qingrun Sibian decoction(清 润 四 边 汤),an external application formula,for vulvar lichen sclerosus through network pharmacology and molecular docking techniques.Methods:The Traditional Chinese Medicine Systems Pharmacology Platform(TCMSP)and the HERB database were utilized to screen the active components and targets of the main drugs in the Qingrun Sibian decoction.The Human Gene Information Database(GeneCards)and the Online Mendelian Inheritance in Man(OMIM)platform were referenced to identify disease targets.Following the intersection with disease target genes,a protein-protein interaction(PPI)network was established.The DAVID database was employed for Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of the potential core target genes.Molecular docking was applied to validate the molecular binding capacity between the bioactive components of Qingrun Sibian decoction and the core targets.Results:A total of 166 active components and 597 targets of Qingrun Sibian decoction were screened out.The key components included quercetin,luteolin,apigenin,kaempferol,and norartocarpetin.The core genes were tumor suppressor factor(TP53),ESR1 encoding estrogen receptor(ERα),anti-apoptotic protein(BcL-2),epidermal growth factor receptor(EGFR),and human epidermal growth factor receptor 2(ERBB2).The treatment of vulvar lichen sclerosus was achieved by regulating endocrine resistance,cancer signaling pathways,estrogen signaling pathways,and EGFR tyrosine kinase inhibitor resistance pathways.The molecular docking results showed that the active components of Qingrun Sibian decoction had strong affinity with the core target genes.Conclusion:The external application of Qingrun Sibian decoction can effectively inhibit the immune-mediated inflammatory responses and promote normal immune system functioning via multiple active components and pathways,thereby achieving the therapeutic goal for vulvar lichen sclerosus.关键词
清润四边汤/外阴硬化性苔藓/网络药理学/分子对接Key words
Qingrun Sibian decoction/Vulvar lichen sclerosus/Network pharmacology/Molecular docking分类
医药卫生引用本文复制引用
郑奕宁,顾燕频..基于网络药理学与分子对接技术探索清润四边汤外用治疗外阴硬化性苔藓的作用机制研究[J].中医临床研究,2026,18(19):48-58,11.基金项目
浙江省"小而强"临床创新团队项目(CXTD202501010) （CXTD202501010）
浙江省中医药重点学科建设项目(2024-XK-07) （2024-XK-07）
浙江省中医药科技计划项目(2022ZA035) （2022ZA035）
浙江省中青年临床名中医项目(浙中医药[2021]22号). （浙中医药[2021]22号）
