中医临床研究2026，Vol.18Issue(20)：1-8,8.DOI:10.3969/j.issn.1674-7860.2026.20.001
"调神畅志"针法对左旋多巴诱发异动症大鼠纹状体细胞外信号调节激酶/哺乳动物雷帕霉素靶蛋白信号通路调控的影响
Influence of"Tiaoshen Changzhi"acupuncture therapy on the regulation of ERK/mTOR pathway in striatum of rats with levodopa-induced dyskinesia
摘要
Abstract
Objective:To observe the effect of"Tiaoshen Changzhi(调神畅志)"acupuncture therapy on improving the symptoms of levodopa-induced transactivity disorder(LID)rats,and explore the mechanism of the ERK/mTOR signaling pathway.Methods:Parkinson's disease(PD)model was established by stereotaxic intracerebral injection of 6-OHDA,and LID model was further induced by intraperitoneal injection of levodopa/benzylserazid.Six groups:normal group,sham operation group,model group,Western medicine group,Tiaoshen Changzhi group and ordinary acupuncture group.Tiaoshen Changzhi group selected the following acupoints"Baihui(GV20)through to Taiyang(EX-HN5)","Yintang(EX-HN3)","Zhongwan(CV12)","Juque(CV14)",bilateral"Three Yin Jiao(SP6)",bilateral"Taichong(LR3)".The ordinary acupuncture group selected the"dance tremor control area",bilateral"Fengchi(GB2)",bilateral"Hegu(LI4)",bilateral"Taichong(LR3)"and bilateral"Yanglingquan(GB34)".Apomorphine(APO)induced behavioral assessment,abnormal involuntary movement scale(AIM)score,left forelimb function evaluation and the contralateral rotation test were used to observe the behavioral changes in each group after modeling and in each group after intervention.The expression of mTOR and ERK in rat striatal tissue was measured by immunohistochemistry.The expression of mTOR mRNA and ERK mRNA in rat striatal tissue was quantified by qRT-PCR.Results:The contralateral rotation behavior and AIM score of the Western medicine group,the ordinary acupuncture group and Tiaoshen Changzhi group were significantly decreased compared with the model group(P<0.01),and the Western medicine group and Tiaoshen Changzhi group were significantly lower than those in the ordinary acupuncture group(P<0.05).Compared with model group,the relative expressions of ERK,mTOR proteins and ERK mRNA,mTOR mRNA in striatal tissue of rats in Tiaoshen Changzhi group,the ordinary acupuncture group and Western medicine group were significantly decreased(P<0.01),and the Western medicine group was lower than the Tiaoshen Changzhi group(P<0.05),while the Tiaoshen Changzhi group was lower than the ordinary acupuncture group(P<0.05).Conclusion:"Tiaoshen Changzhi"acupuncture can improve the behavioral performance of LID rats,reduce abnormal involuntary movement and contralateral rotation behavior,and its mechanism may be related to reducing the expression levels of ERK and mTOR proteins in the striatum of LID rats and inhibiting the ERK/mTOR signaling pathway.关键词
左旋多巴诱发异动症/"调神畅志"针刺/纹状体/细胞外信号调节激酶/哺乳动物雷帕霉素靶蛋白通路Key words
Levodopa-induced dyskinesia/"Tiaoshen Changzhi"acupuncture/Striatum/ERK/mTOR signaling pathway分类
医药卫生引用本文复制引用
秦正巍,梁志明.."调神畅志"针法对左旋多巴诱发异动症大鼠纹状体细胞外信号调节激酶/哺乳动物雷帕霉素靶蛋白信号通路调控的影响[J].中医临床研究,2026,18(20):1-8,8.基金项目
广州市科技计划项目(2023A03J0775) （2023A03J0775）
广东省针灸学会科研课题(GDZJ2024010) （GDZJ2024010）
广州市卫健委项目(20241A010085). （20241A010085）
