中药新药与临床药理2026，Vol.37Issue(9)：1675-1684,10.DOI:10.19378/j.issn.1003-9783.2026.09.007
益气解毒方下调PTGES2抑制鼻咽癌细胞迁移与侵袭的机制
Study on the Mechanism of Yiqi Jiedu Prescription Inhibiting Migration and Invasion of Nasopharyngeal Carcinoma Cells by Downregulating PTGES2
摘要
Abstract
Objective To investigate the effects of Yiqi Jiedu Prescription(YJP)and prostaglandin E synthase 2(PTGES2)on the migration and invasion of nasopharyngeal carcinoma(NPC)5-8F cells,as well as the role of PTGES2 in the effects of YJP.Methods(1)5-8F cells were divided into a control group,a low-concentration YJP group(0.5 mg·mL-1),a high-concentration YJP group(1.0 mg·mL-1),and a cisplatin group(2 µg·mL-1).Wound healing assay and Transwell invasion assay were used to assess cell migration and invasion abilities.The protein expression levels of PTGES2,Vimentin,N-cadherin,and E-cadherin were determined by Western Blot.(2)5-8F cells were transduced with lentiviral vectors for PTGES2 overexpression(oe-PTGES2)and the corresponding negative control(oe-NC),or for PTGES2-targeting shRNA(sh-PTGES2)and its negative control(sh-NC).The transduced cells were treated with or without YJP(1.0 mg·mL-1).For the overexpression experiments,cells were divided into the oe-NC group,oe-NC+YJP group,oe-PTGES2 group,and oe-PTGES2+YJP group.For the knockdown experiments,cells were divided into the sh-NC group,sh-NC+YJP group,sh-PTGES2 group,and sh-PTGES2+YJP group.Wound healing assay and Transwell invasion assay were used to assess cell migration and invasion abilities,and Western Blot was performed to measure the protein expression levels of PTGES2,Vimentin,phosphorylated AKT(p-AKT),N-cadherin,and E-cadherin.Results(1)Compared with the control group,the high-concentration YJP group and the cisplatin group exhibited significantly lower relative migration and invasion rates(P＜0.01),reduced protein expression of PTGES2,Vimentin,and N-cadherin,and increased expression of E-cadherin(P＜0.05,P＜0.01).(2)After lentiviral transduction,compared with the oe-NC group,the oe-PTGES2 group significantly enhanced relative migration and invasion rates(P＜0.01),along with increased protein expression of PTGES2,Vimentin,p-AKT,and N-cadherin(P＜0.05,P＜0.01),and decreased E-cadherin expression(P＜0.05).Compared with the sh-NC group,the sh-PTGES2 group exhibited reduced relative migration and invasion rates(P＜0.01),downregulated protein expression of PTGES2,Vimentin,p-Akt,and N-cadherin(P＜0.01),and upregulated E-cadherin expression(P＜0.01).When combined with YJP treatment,compared with the oe-NC+YJP group,the relative migration and invasion rates of cells in the oe-PTGES2+YJP group were increased(P＜0.01),the protein expression of p-Akt,and N-cadherin were elevated(P＜0.05,P＜0.01),and the expression of E-cadherin was decreased(P＜0.05).In contrast,compared with the sh-NC+YJP group,the sh-PTGES2+YJP group showed reduced relative migration and invasion rates(P＜0.01),decreased protein expression of PTGES2,Vimentin,p-Akt,and N-cadherin(P＜0.01),and increased E-cadherin expression(P＜0.01).Conclusion PTGES2 can promote the migration and invasion of NPC cells;the inhibitory effect of YJP on the migration and invasion of NPC cells may involve suppression of the PTGES2/AKT pathway.关键词
益气解毒方/鼻咽癌/前列腺素E合酶2(PTGES2)/迁移/侵袭/5-8F细胞Key words
Yiqi Jiedu Prescription/nasopharyngeal carcinoma/PTGES2/migration/invasion/5-8F cells分类
医药卫生引用本文复制引用
刘思颖,张文青,刘洁,王贤文,何迎春..益气解毒方下调PTGES2抑制鼻咽癌细胞迁移与侵袭的机制[J].中药新药与临床药理,2026,37(9):1675-1684,10.基金项目
国家自然科学基金项目(82505683) （82505683）
湖南省自然科学基金项目(2023JJ30449,2023JJ30475) （2023JJ30449,2023JJ30475）
湖南省卫生健康委员会项目(20257618) （20257618）
湖南中医药大学研究生科研创新项目(2025CX032,CX20251197). （2025CX032,CX20251197）
