中国临床药理学杂志2026，Vol.42Issue(16)：2330-2336,7.DOI:10.13699/j.cnki.1001-6821.2026.16.013
黄柏碱调节AMPK/FOXO3a信号通路对非小细胞肺癌细胞增殖、侵袭和上皮间质转化影响的研究
Reserch of the effects of phellodendrine adjusting the AMPK/FOXO3a signaling pathway on the proliferation,invasion and epithelial-mesenchymal transition of non-small cell lung cancer cells
摘要
Abstract
Objective To explore the effects of phellodendrine adjusting the AMP-activated protein kinase(AMPK)/forkhead box protein O3(FOXO3a)signaling pathway on the proliferation,invasion and epithelial-mesenchymal transition of non-small cell lung cancer cells.Methods Human non-small cell lung cancer cell line NCI-H1650 was divided into control group,low-dose experimental group,medium dose experimental group,high-dose experimental group,and an inhibitor group.The control group was cultured in medium containing an equal volume of phosphate-buffered saline(PBS).The low-dose experimental group,the medium dose experimental group,and the high-dose experimental group were cultured in media containing 10,20,and 40 μ mol·L-1 berberine,respectively.The inhibitor group was cultured in media containing 40 μ mol·L-1 berberine and 10 μ mol·L-1 Dorsomorphin.Use 5-Ethynyl-2′-deoxyuridine(EdU)to detect cell proliferation ability,use flow cytometry to detect cell apoptosis ability,use Transwell to detect cell migration and invasion ability,use Western blot to detect the relative expression levels of epithelial mesenchymal transition proteins E-cadherin,N-cadherin,vimentin,and AMPK/FOXO3a pathway proteins.Results The EdU positive rates of the control group,low-dose experimental group,medium dose experimental group,high-dose experimental group,and inhibitor group were(37.95±4.57)％,(30.14±3.73)％,(22.86±2.95)％,(15.34±1.97)％and(33.48±4.02)％,respectively;the cell apoptosis rates were(2.84±0.39)％,(9.46±1.25)％,(16.32±1.97)％,(23.01±2.74)％and(5.98±0.86)％,respectively;the number of invasive cells were(73.17±8.89),(58.00±6.87),(43.67±5.54),(29.83±3.97)and(67.17±7.73),respectively;E-cadherin protein expression were 1.05±0.12,1.35±0.16,1.64±0.19,1.92±0.23 and 1.14±0.14,respectively;N-cadherin protein expression were 1.03±0.11,0.79±0.09,0.56±0.07,0.34±0.05 and 0.95±0.12,respectively;Vimentin protein expression were 1.06±0.12,0.82±0.10,0.58±0.08,0.35±0.06 and 0.97±0.13,respectively;the phosphorylated(p)-AMPK/AMPK levels were 0.38±0.05,0.55±0.07,0.71±0.09,0.88±0.10 and 0.41±0.05,respectively;the p-FOXO3a/FOXO3a levels were 0.33±0.04,0.51±0.06,0.68±0.08,0.85±0.09 and 0.35±0.04,respectively.There were statistically significant differences in the EdU positivity rate,migration and invasion cell numbers,apoptosis rate,E-cadherin,N-cadherin and Vimentin protein expression,as well as p-AMPK and p-FOXO3a levels of NCI-H1650 cells between the low-dose experimental group,medium dose experimental group,high-dose experimental group,and inhibitor group.Conclusion Phellodendrine inhibits the proliferation,invasion and epithelial-mesenchymal transition of non-small cell lung cancer cells,and promotes apoptosis by activating the AMPK/FOXO3a signaling pathway.关键词
黄柏碱/AMP活化蛋白激酶/叉头盒蛋白O3/非小细胞肺癌/增殖/侵袭/上皮间质转化Key words
phellodendrine/AMP-activated protein kinase/forkhead box protein O3/non-small cell lung cancer/proliferation/invasion/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
任维敏,张宝,张国臣,乔鹏涛..黄柏碱调节AMPK/FOXO3a信号通路对非小细胞肺癌细胞增殖、侵袭和上皮间质转化影响的研究[J].中国临床药理学杂志,2026,42(16):2330-2336,7.基金项目
济南市卫生健康委员会科技发展计划基金资助项目(2024302016) （2024302016）
