中国临床药理学杂志2026，Vol.42Issue(16)：2351-2356,6.DOI:10.13699/j.cnki.1001-6821.2026.16.016
蛇床子素调节JAK2/STAT3信号通路对乙型肝炎病毒大鼠肝功能及肝纤维化影响的研究
Research of the effects of osthole on liver function and liver fibrosis in hepatitis B virus rats by modulating the JAK2/STAT3 signaling pathway
摘要
Abstract
Objective To investigate the effects of osthole(Ost)adjusting the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)pathway on liver function and liver fibrosis in hepatitis B virus(HBV)rats.Methods An HBV rat model was established.The successfully modeled rats were divided into model(normal saline),low-(10 mg·kg-1 Ost),medium-(20 mg·kg-1 Ost)and high-dose(40 mg·kg-1 Ost)experimental groups,JAK2 pathway activator group(40 mg·kg-1 Ost+4.0 mg·kg-1 Coumermycin A1)with 12 rats in each group,and another 12 rats were selected as blank control group(normal saline).Liver function indicators were detected using an automatic biochemical analyzer.Inflammatory cytokines,fibrotic markers,and levels of hepatitis B surface antigen(HBsAg)and hepatitis B e antigen(HBeAg)were measured by enzyme-linked immunosorbent assay(ELISA).Hepatitis B virus deoxyribonucleic acid(HBV-DNA)content was detected by real-time quantitative polymerase chain reaction(real-time qPCR).Hematoxylin-eosin(HE)staining was used to observe pathological changes in liver tissue,and Masson staining was used to observe liver fibrosis.Western blot was employed to detect the expression of JAK2/STAT3 pathway-related proteins.Results The levels of alanine aminotransferase(ALT)in the blank control group,model group,low-,medium-,and high-dose experimental groups and JAK2 pathway activator group were(30.15±6.29),(78.56±7.92),(62.33±7.16),(50.08±6.88),(37.89±6.54)and(70.46±7.53)U·L-1,respectively;the levels of aspartate aminotransferase(AST)were(68.52±10.63),(115.37±12.03),(102.65±11.54),(85.97±11.19),(72.11±10.87)and(109.83±11.66)U·L-1,respectively;the levels of interleukin-6(IL-6)were(62.78±11.94),(146.32±14.76),(121.53±13.05),(95.67±12.79),(70.29±12.32)and(135.62±13.82)pg·mL-1,respectively;the levels of HBsAg were(0.00±0.00),(3120.54±320.16),(2 436.83±296.51),(1 653.28±281.03),(1 026.49±273.64)and(2 903.66±308.59)U·mL-1,respectively;the ratios of phosphorylated(p)-JAK2/JAK2 were 0.29±0.04,0.77±0.09,0.63±0.07,0.50±0.06,0.35±0.05 and 0.72±0.08,respectively;the ratios of p-STAT3/STAT3 were 0.41±0.04,0.86±0.09,0.72±0.07,0.59±0.06,0.46±0.05 and 0.79±0.08,respectively.Comparisons between the model group and the blank control group,between the low-,medium-,and high-dose experimental groups and the model group,and between the JAK2 pathway activator group and the high-dose experimental group all showed statistically significant differences in the above indicators(all P＜0.05).Conclusion Ost may inhibit liver fibrosis in HBV rats and improve liver function by suppressing the JAK2/STAT3 pathway.关键词
蛇床子素/Janus激酶2/信号转导和转录激活因子3通路/乙型肝炎病毒/肝功能/肝纤维化Key words
osthole/Janus kinase 2/signal transducer and activator of transcription 3 pathway/hepatitis B virus/liver function/liver fibrosis分类
医药卫生引用本文复制引用
唐莉莉,王雪,张盼,宋惠,王治勇..蛇床子素调节JAK2/STAT3信号通路对乙型肝炎病毒大鼠肝功能及肝纤维化影响的研究[J].中国临床药理学杂志,2026,42(16):2351-2356,6.基金项目
浦东新区卫生系统重点学科群项目(PWZxq2022-08) （PWZxq2022-08）
上海市浦东新区卫健委联合攻关项目(PW2023D-01) （PW2023D-01）
上海市科技计划基金资助项目(24SF1903600) （24SF1903600）
