中国药理学通报2026，Vol.42Issue(10)：1950-1959,10.DOI:10.12360/CPB202601010
基于网络药理学和体外实验探究山银花活性成分防治血管再狭窄的作用机制
Mechanism of action of active ingredients of Lonicerae Flos in preventing and treating in-stent restenosis based on network pharmacology and in vitro experiments
摘要
Abstract
Aim To explore the effect and specific mechanism of active ingredients from Lonicera macran-thoides in preventing and treating in-stent restenosis(ISR)based on network pharmacology and in vitro ex-periments.Methods Active ingredients and potential targets of L.macranthoides for ISR treatment were identified via network pharmacology.Following the construction of a protein-protein interaction(PPI)net-work,gene ontology(GO)functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed on the intersecting targets to further investigate the regulatory role and mechanism of L.macranthoides active ingredients in ISR.Molecular docking was then conducted between the active ingredients and key targets.Additionally,a VSMC abnormal proliferation model was established by inducing vascular smooth muscle cells(VSMCs)with PDGF-BB.Cell viability and proliferation were assessed using the CCK-8 assay and EdU assay,re-spectively,while cell migration was evaluated via scratch assay.Immunofluorescence and Western blot were used to detect PCNA protein expression and the expression of proteins related to the phosphatidylinosi-tol 3-kinase(PI3K)/protein kinase B(Akt)pathway.Results A total of 31 active ingredients from L.mac-ranthoides were screened,primarily including dipsaco-side B(DB),lonicerin,isoquercetin,caffeic acid,chlorogenic acid,etc.,involving 527 targets,with 138 overlapping targets related to ISR.GO and KEGG enrichment analyses indicated that these targets were mainly associated with the PI3K/Akt signaling path-way,cancer pathways,cellular response to external survival and growth signals,lipid and atherosclerosis signaling pathways,MAPK-related pathways,etc.Key core targets included TNF,AKT1,STAT3,PTGS2,MMP9,among others.Molecular docking re-sults demonstrated that DB,an important active ingre-dient of L.macranthoides,bound well to core targets.Cell experiments revealed that DB significantly inhib-ited PDGF-BB-induced VSMC proliferation and migra-tion,and downregulated the expression of PCNA,p-PI3K,and p-Akt proteins.Conclusion L.macran-thoides inhibits the occurrence and development of ISR through multi-component,multi-target,and multi-pathway synergistic effects,providing new insights for further elucidating its mechanism in treating ISR.关键词
山银花/血管再狭窄/活性成分/网络药理学/血管平滑肌细胞/作用机制Key words
Lonicerae Flos/in-stent restenosis/ac-tive ingredients/network pharmacology/vascular smooth muscle cells/mechanism of action分类
医药卫生引用本文复制引用
杨冬梅,韦晶玥,李鎏,陈惠卿,吴可青,李浩民,庹勤慧..基于网络药理学和体外实验探究山银花活性成分防治血管再狭窄的作用机制[J].中国药理学通报,2026,42(10):1950-1959,10.基金项目
国家自然科学基金资助项目(No 82474115) （No 82474115）
湖南省自然科学基金项目(No 2025JJ90080) （No 2025JJ90080）
湖南省科技创新计划项目(No 2021RC4064) （No 2021RC4064）
湖南省教育厅一般项目(No 25C0235) （No 25C0235）
湖南省中医药管理局项目(No B20260015) （No B20260015）
